Document Detail


Investigation of ternary complex formations of polyacrylic acid with bovine serum albumin in the presence of metal ions by fluorescence and dynamic light scattering measurements.
MedLine Citation:
PMID:  20556638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, the complex formation of bovine serum albumin (BSA) and polyacrylic acid (PAA) in the presence metal ions at pH = 7 has been examined by using fluorescence and dynamic light scattering measurements. It has been observed that the most stable complexes of polyacrylic acid and bovine serum albumin have occurred in the presence of copper(II) ions. The other ions have the ability to form weak complexes between polyions and bovine serum albumin. To prior characterizing the interaction between bovine serum albumin and polyacrylic acid, the dynamic light scattering technique have been applied to determine the intensity-size distributions of the solutions of bovine serum albumin, polyacrylic acid, and ternary mixtures containing various molar ratios of bovine serum albumin to polyacrylic acid (the molar ratios of bovine serum albumin to polyacrylic acid has been taken equal to 0.5, 1.0, 1.5, 2.0 and 2.5) prepared at different molar ratios of copper(II) ions/acrylic acid unit. When the molar ratio of copper(II) ions to acrylic acid in the ternary mixtures has been lower than and equals to 0.3, two peaks have been observed in the curves of the intensity-size distributions due to contents of free bovine serum albumin and ternary complexes of polyacrylic acid-copper(II)-bovine serum albumin whereas when the molar ratio of copper(II) ions to acrylic acid equals to 0.4, the hydrodynamic diameter has pointed out only one peak. This result indicates that soluble and stable ternary complexes has occurred when the molar ratio of copper(II) ions to acrylic acid has been taken equal to 0.4.
Authors:
Mesut Karahan; Zeynep Mustafaeva; Cemal Ozeroğlu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The protein journal     Volume:  29     ISSN:  1875-8355     ISO Abbreviation:  Protein J.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101212092     Medline TA:  Protein J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  336-42     Citation Subset:  IM    
Affiliation:
Faculty of Arts and Sciences, Department of Chemistry, Yildiz Technical University, Esenler, Istanbul, Turkey. mesut.karahan@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / chemistry*,  metabolism
Animals
Cattle
Copper / chemistry*,  metabolism
Light
Models, Chemical*
Multiprotein Complexes / chemistry*,  metabolism
Protein Multimerization
Scattering, Radiation
Serum Albumin, Bovine / chemistry*,  metabolism
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Multiprotein Complexes; 0/Serum Albumin, Bovine; 7440-50-8/Copper; 9003-01-4/carbopol 940

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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