Document Detail


Investigation of Hypoxia-induced Myocardial Injury Dynamics in a Tissue Interface Mimicking Microfluidic Device.
MedLine Citation:
PMID:  23205467     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Myocardial infarction is a major cause of morbidity and mortality worldwide. However, the methodological development of a spatiotemporally controllable investigation of the damage events in myocardial infarction remains challengeable. In the present study, we describe a micropillar array-aided tissue interface mimicking microfluidic device for the dynamic study of hypoxia-induced myocardial injury in a microenvironment-controllable manner. The mass distribution in the device was visually characterized, calculated, and systematically evaluated using the micropillar-assisted biomimetic interface, physiologically relevant flows, and multitype transportation. The fluidic microenvironment in the specifically functional chamber for cell positioning and analysis was successfully constructed with high fluidic relevance to the myocardial tissue. We also performed a microenvironment-controlled microfluidic cultivation of myocardial cells with high viability and regular structure integration. Using the well-established culture device with a tissue-mimicking microenvironment, a further on-chip investigation of hypoxia-induced myocardial injury was carried out, and the varying apoptotic responses of myocardial cells were temporally monitored and measured. The results show that the hypoxia directionally resulted in observable cell shrinkage, disintegration of the cytoskeleton, loss of mitochondrial membrane potential, and obvious activation of Caspase-3, which indicates its significant apoptosis effect on myocardial cells. We believe this microfluidic device can be suitable for temporal investigations of cell activities and responses in myocardial infarction. It is also potentially valuable to the micro-control development of tissue-simulated studies of multiple clinical organ/tissue disease dynamics.
Authors:
Li Ren; Wenming Liu; Yaolei Wang; Jian-Chun Wang; Qin Tu; Xu Juan; Rui Liu; Shaofei Shen; Jinyi Wang
Related Documents :
17534137 - Placement of intraventricular catheters using flexible electromagnetic navigation and a...
23332597 - Importance of three-dimensional geometric analysis in the assessment of the athlete's h...
23284897 - Circulating annexin a5 levels after atrial switch for transposition of the great arteri...
16208697 - Coronary embolization of a gauze fragment: a cautionary case report.
22361987 - Intra-aortic balloon pump for hemodynamic support in hepatobiliary surgery: report of a...
2038067 - Preserving effect of fructose-1,6-bisphosphate on high-energy phosphate compounds durin...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The nature of goal-directed action representations in infancy.
Next Document:  Resiquimod, a topical drug for viral skin lesions and skin cancer.