Document Detail


Investigation of HSV-1, HSV-2, CMV, HHV-6 and HHV-8 DNA by real-time PCR in surgical resection materials of epilepsy patients with mesial temporal lobe sclerosis.
MedLine Citation:
PMID:  17804017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The objective of this study is to investigate the presence of viral DNAs of HSV-1, HSV-2, HHV-6, HHV-8, and CMV in hippocampus of the patients with mesial temporal lobe epilepsy (MTLE) syndrome. METHODS: Pathological specimens were obtained from 33 patients with MTLE undergone temporal lobectomy with amygdalo-hippocampectomy due to intractable seizures. Autopsy materials from the hippocampus of 7 patients without neurological disease were used as controls. The data was also correlated with the clinical history of patients including febrile convulsions, age, and history of CNS infections. Real-time polymerase chain reaction method was performed for detection of DNAs of these viruses. RESULTS: HHV-6, HSV-1 and HHV-8 were detected in the hippocampus of 3, 2 and 1 patients with MTLE respectively. None of the hippocampus of patients with MTLE was positive for DNA of HSV-2 and/or CMV. Three patients with positive HHV-6 DNAs had febrile convulsions and family history for epilepsy. None of our control specimens showed PCR positivity to any of the 5 tested viruses. CONCLUSIONS: Our study is the first to report the presence of HHV-8 viral genome in the brain tissue of patient with MTLE. Viral DNAs were detected in a total of 18% of the patients in this study; we can conclude that activity of the latent virus in patients with hippocampal sclerosis should be more extensively studied to establish its role in active infection.
Authors:
Hulya Karatas; Gunfer Gurer; Ahmet Pinar; Figen Soylemezoglu; Gaye Guler Tezel; Gulsen Hascelik; Nejat Akalan; Serdar Tuncer; Abdurrahman Ciger; Serap Saygi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-05
Journal Detail:
Title:  Journal of the neurological sciences     Volume:  264     ISSN:  0022-510X     ISO Abbreviation:  J. Neurol. Sci.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-07     Completed Date:  2008-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375403     Medline TA:  J Neurol Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  151-6     Citation Subset:  IM    
Affiliation:
Hacettepe University, Faculty of Medicine, Department of Neurology, Sihhiye, Ankara, 06100, Turkey. hulyak@hacettepe.edu.tr
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Autopsy
Cytomegalovirus / genetics
DNA, Viral / analysis*
Encephalitis, Herpes Simplex / complications,  diagnosis,  genetics
Epilepsy, Temporal Lobe / pathology,  physiopathology,  virology*
Female
Herpes Simplex / complications
Herpesviridae Infections / complications*,  diagnosis,  genetics*
Herpesvirus 1, Human / genetics
Herpesvirus 2, Human / genetics
Herpesvirus 6, Human / genetics
Herpesvirus 8, Human / genetics*
Hippocampus / pathology,  physiopathology,  virology*
Humans
Male
Reverse Transcriptase Polymerase Chain Reaction
Virus Latency / genetics
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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