Document Detail


Investigation of Enzymatic Hydrolysis Condition on the Properties of Protein Hydrolysate from Fish Muscle (Collichthys niveatus) and Evaluation of Its Functional Properties.
MedLine Citation:
PMID:  22530872     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This study was carried out to investigate the enzymatic hydrolysis condition on the properties of protein hydrolysate from fish muscle of the marine fish species Collichthys niveatus. About 160 fish samples were tested and the analyzed fish species was found to be a lean fish with low fat (1.77 ± 0.01%) and high protein (16.76 ± 1.21%). Fish muscle of C. niveatus was carefully collected and hydrolyzed with four commercial enzymes: Alcalase, Neutrase, Protamex, and Flavourzyme under the conditions recommended by the manufacturers. Among the tested proteases, the Neutrase catalyzed the hydrolysis process most effectively since the hydrolysate generated by Neutrase have the highest content of sweet and umami taste amino acids (SUA). The effect of hydrolysis conditions was further optimized using response surface methodology (RSM), and the optimum values for temperature, pH, and enzyme/substrate ratio (E/S ratio), were found to be 40.7 ◦C, 7.68, and 0.84%, respectively. Finally, the amino acid composition of the hydrolysate was analyzed by AccQ•Tag derivatization and HPLC-PDA determination. Major amino acids of muscle of C. niveatus were Threonine, Glutamic acid, Phenyalanine, Tryptophan and Lysine, accounting for respectively 10.92%, 10.85%, 10.79%, 9.86%, and 9.76% of total amino acid content. The total content of essential amino acids was 970.7 ng•mL-1, while that of non-essential amino acids was 709.1 ng•mL-1. The results suggest that the fish muscle and its protein hydrolysate from C. niveatus provide versatile supply of the benefits and can be incorporated as supplement in health-care food.
Authors:
Qing Shen; Rui Guo; Zhiyuan Dai; Yanping Zhang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-24
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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