Document Detail

An Investigation of the Early Detection of Radiation Induced Apoptosis by <sup>99m</sup>Tc-Annexin V and <sup>201</sup>Thallium-Chloride in a Lung Cancer Cell Line.
MedLine Citation:
PMID:  22739005     Owner:  NLM     Status:  In-Data-Review    
This study aims to investigate the efficacy of in vitro Thallium-201 Chloride (Tl-201) and in vitro and in vivo Tc-99m HYNIC-coupled Annexin V (TAV) in the early detection of radiation induced apoptosis, a proxy indicator of radiation therapy (RT) efficacy. In vitro Tl-201 and TAV accumulation and efflux in non-small cell lung cancer were measured post irradiation at 5 different gamma ray doses. The replication rates (RR) of the cell lines were also measured. The same non-small cell lung cancer line was inoculated into the left femur. In vivo non-invasive Tl-201 and TAV tracer biodistribution studies were performed. Cell RR decrease with increased radiation dose was observed 48 hours after irradiation. Apoptotic cell number was found to have increased in response to 9 Gy and 12 Gy radiation dose. Tl-201 accumulation in the 9 Gy and 12 Gy irradiation groups was found to be higher than the lower irradiation groups. Quick Tl-201 efflux was observed in the 9 Gy and 12 Gy irradiated cells. At 48 hours after irradiation with 9 Gy and 12 Gy, Annexin V accumulation was found to be higher than in the control and 3-6 Gy groups. In vivo mouse model confirmed the increased TAV uptake in implanted tumors for relatively high 9 Gy irradiation as compared to non-irradiated controls. TAV may prove to be an effective radiotracer for early assessment of radiation therapy efficacy, via apoptosis, in human lung cancers.
Mahbub E Khoda; Keita Utsunomiya; Sangkil Ha-Kawa; Shohei Kanno; Yumiko Kono; Satoshi Sawada
Related Documents :
2072815 - Insulin-like growth factor i enhances proenkephalin synthesis and dopamine beta-hydroxy...
6939935 - Characteristics of the chemotactic factor-mediated cell swelling response of tumor cells.
12074575 - Effects of a non-igf binding mutant of igfbp-5 on cell death in human breast cancer cells.
23704865 - Correction: the endocytic adaptor eps15 controls marginal zone b cell numbers.
20506405 - Gab1 mediates hepatocyte growth factor-stimulated mitogenicity and morphogenesis in mul...
12673595 - Glutamylated tubulin: diversity of expression and distribution of isoforms.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of radiation research     Volume:  53     ISSN:  1349-9157     ISO Abbreviation:  J. Radiat. Res.     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376611     Medline TA:  J Radiat Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  361-7     Citation Subset:  IM    
Department of Radiology, Kansai Medical University.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Protective Effect of Polysaccharides Isolated from <i>Tremella fuciformis</i> against Radiation-indu...
Next Document:  Radioprotective Effects of ON 01210.Na upon Oral Administration.