Document Detail


Investigation of a Cu(II)-poly(gamma-glutamic acid) complex in aqueous solution and its insulin-mimetic activity.
MedLine Citation:
PMID:  17429807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The complexation between cupric ions (Cu(II)) and poly(gamma-glutamic acid) (gamma-PGA) in aqueous solutions (pH 3-11) has been studied by UV-visible absorption and electron spin resonance (ESR) techniques. Formation of the Cu(II)-gamma-PGA complex is confirmed by the observation of the blue shift of the absorption band in the visible region, anisotropic line shapes in the ESR spectrum at room temperature, and a computer simulation of the visible absorption spectrum of the complex. The structure of the Cu(II)-gamma-PGA complex, depending on the pH, has been determined. The in vitro insulin-mimetic activity of the Cu(II)-gamma-PGA complex is examined by determining both inhibition of free fatty acid release and glucose uptake in isolated rat adipocytes treated with epinephrine, in which the concentration of the Cu(II)-gamma-PGA complex for 50% inhibition of free fatty acid release is very similar to that of CuSO4. However, it is significantly lower than that of a previously reported insulin-mimetic bis(3-hydroxypicolinato)copper(II), [Cu(3hpic)2], complex.
Authors:
Subarna Karmaker; Tapan K Saha; Hiromu Sakurai
Related Documents :
3676247 - Involvement of the solid phase of rumen digesta in the interaction between copper, moly...
18964197 - Stability constants and molar absorptivities for complexes of copper(ii) with n-methyld...
17182387 - Treatment of wastewater containing cu(ii)-edta using immobilized tio2/solar light.
11803037 - Roles of metallothionein in copper homeostasis: responses to cu-deficient diets in mice.
19670837 - Architectures of strontium hydroxyapatite microspheres: solvothermal synthesis and lumi...
21202927 - N'-(5-bromo-2-methoxy-benzyl-idene)-3-hydroxy-benzohydrazide methanol hemisolvate.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Macromolecular bioscience     Volume:  7     ISSN:  1616-5187     ISO Abbreviation:  Macromol Biosci     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-17     Completed Date:  2007-10-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101135941     Medline TA:  Macromol Biosci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  456-66     Citation Subset:  IM    
Affiliation:
Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology,  secretion*
Animals
Biomimetic Materials / chemistry,  pharmacology*
Cells, Cultured
Copper / chemistry,  pharmacology*
Copper Sulfate / pharmacology
Dose-Response Relationship, Drug
Fatty Acids / secretion*
Hydrogen-Ion Concentration
Hypoglycemic Agents / chemistry,  pharmacology*
Insulin*
Male
Polyglutamic Acid / analogs & derivatives*,  chemistry,  pharmacology
Rats
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Hypoglycemic Agents; 0/poly(gamma-glutamic acid); 11061-68-0/Insulin; 25513-46-6/Polyglutamic Acid; 7440-50-8/Copper; 7758-98-7/Copper Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Synthesis and characterization of degradable p(HEMA) microgels: use of acid-labile crosslinkers.
Next Document:  Efficient modification on PLLA by ozone treatment for biomedical applications.