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Investigation of Cell-Substrate Interactions by Focused Ion Beam (FIB) Preparation and Scanning Electron Microscopy (SEM).
MedLine Citation:
PMID:  21345385     Owner:  NLM     Status:  Publisher    
Cell-substrate interactions, which are an important issue in tissue engineering, are studied by using focused ion beam (FIB) milling and scanning electron microscopy (SEM) investigations. Sample cross-sections are generated on predefined positions (target preparation), to investigate the interdependency of growing cells with the substrate material. The experiments focus on two cell culturing systems, hepatocytes (HepG2) on nanoporous aluminum oxide (alumina) membranes and mouse fibroblasts (L929) as well as primary nerve cells on silicon chips with microneedles. On these soft/hard hybrid systems, cross-sections cannot be prepared by conventional techniques like microtomy. Morphological investigations of hepatocytes growing on nanoporous alumina membranes demonstrate that there is an ingrowth of microvilli from the cell surfaces into porous membranes having pore diameters larger than 200 nm. Furthermore, at various cell cultures on the microneedle arrays, the contact between the cells and the microneedles can be observed with high resolution. Based on FIB milled cross-sections and SEM micrographs, it can be clearly distinguished between cells which are only in contact with microneedles and cells which are penetrated by microneedles. Especially, target preparation of biological samples with FIB technique offers the possibility to prepare not only soft material but also hybrid samples (soft/hard materials). Followed by high resolution imaging with SEM, new insights into cell surface interactions can be received.
Andrea Friedmann; Andreas Hoess; Andreas Cismak; Andreas Heilmann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-20
Journal Detail:
Title:  Acta biomaterialia     Volume:  -     ISSN:  1878-7568     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Department of Biological and Macromolecular Materials Fraunhofer Institute for Mechanics of Materials, Walter-Hülse-Strasse 1, Halle, 06120, Germany.
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