Document Detail

Inverting character of family GH115 α-glucuronidases.
MedLine Citation:
PMID:  20804758     Owner:  NLM     Status:  MEDLINE    
α-Glucuronidases of glycoside hydrolase family 115 of the xylose-fermenting yeast Pichia stipitis and wood-destroying fungus Schizophyllum commune liberate 4-O-methyl-D-glucuronic acid residues from aldouronic acids and glucuronoxylan. The specific activities of both enzymes depended on polymerization degree of the acidic xylooligosaccharides and were inhibited by linear β-1,4-xylooligosaccharides. These results suggest interaction of the enzyme with several xylopyranosyl residues of the xylan main chain. Using (1)H NMR spectroscopy and reduced aldopentaouronic acid (MeGlcA(3)Xyl(4)-ol) as a substrate, it was found that both enzymes are inverting glycoside hydrolases releasing 4-O-methyl-D-glucuronic acid (MeGlcA) as its β-anomer.
Katarína Kolenová; Olena Ryabova; Mária Vrsanská; Peter Biely
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-07
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2010-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  4063-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Bratislava, Slovakia.
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MeSH Terms
Glycoside Hydrolases / chemistry,  metabolism*
Nuclear Magnetic Resonance, Biomolecular
Pichia / enzymology*
Schizophyllum / enzymology*
Substrate Specificity
Wood / microbiology*
Reg. No./Substance:
EC 3.2.1.-/Glycoside Hydrolases; EC

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