Document Detail

Invertebrate hematopoiesis: an astakine-dependent novel hematopoietic factor.
MedLine Citation:
PMID:  21220699     Owner:  NLM     Status:  In-Data-Review    
A novel factor, named crustacean hematopoietic factor (CHF), was identified from a library of suppression subtractive hybridization with the aim to find downstream genes of an invertebrate cytokine, astakine 1, in the freshwater crayfish Pacifastacus leniusculus. CHF is a small cysteine-rich protein (∼9 kDa) with high similarity to the N-terminal region of vertebrate CRIM1 in containing an insulin growth factor binding protein variant motif with unknown function. CHF was found to be induced in primary cell cultures of crayfish hematopoietic tissue (Hpt) cells (precursors of crayfish blood cells) after treatment with astakine 1. Silencing of CHF did not affect the renewal of Hpt cells in vitro, but induced apoptosis of Hpt cells. CHF is exclusively expressed in the blood cell lineage of crayfish (Hpt cells and blood cells), and in vivo RNA interference experiments show that knockdown of this gene results in severe loss of blood cells and a higher apoptotic rate in Hpt. Our data further suggest that crayfish CHF is critical for the survival of hemocytes and Hpt cells by preventing their apoptosis, thus it plays an important role in hemocyte homeostasis in crayfish. Our study of CHF may also shed light on the function of this untypical insulin growth factor binding protein motif located in the N-terminal of vertebrate CRIM1.
Xionghui Lin; Kenneth Söderhäll; Irene Söderhäll
Related Documents :
16709639 - Amp-activated protein kinase and the regulation of ca2+ signalling in o2-sensing cells.
18723529 - Hydrogen sulfide and oxygen sensing: implications in cardiorespiratory control.
222719 - Hydroxyl radical formation in phagocytic cells of the rat.
15299119 - Traffic lights in trichodesmium. regulation of photosynthesis for nitrogen fixation stu...
19337519 - Activated toxicity of diesel particulate extract by ultraviolet a radiation in mammalia...
9337489 - Membrane-linked systems preventing superoxide formation.
24874429 - Rapamycin-resistant parp1 overexpression is a potential therapeutic target in lymphangi...
12412009 - Evidence that nitric oxide regulates cell-cycle progression in the developing chick neu...
4037379 - Comparative effects of halothane, enflurane, and methoxyflurane on the incidence of abn...
Publication Detail:
Type:  Journal Article     Date:  2011-01-10
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  186     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2073-9     Citation Subset:  AIM; IM    
Department of Comparative Physiology, Uppsala University, 752 36 Uppsala, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Tribbles 3: a novel regulator of TLR2-mediated signaling in response to Helicobacter pylori lipopoly...
Next Document:  Air Pollution, Blood Pressure, and the Risk of Hypertensive Complications During Pregnancy: The Gene...