| Inversion of transfer modes and sex factor-chromosome interactions in conjugation in Escherichia coli. | |
| | |
MedLine Citation:
|
PMID: 5335907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A study was made of the mating properties of an unusual system of interconvertible donor strains of Escherichia coli K-12: Ra-1, Ra-2, and RaF(+). The Ra-1 and Ra-2 strains are Hfr strains whose origins are widely separated on the chromosome and whose transfer modes proceed in the opposite direction from one another. When Ra-1 cells were mated with females, a small fraction of the donors transferred markers via the Ra-2 mode. This effect was enhanced by preconjugal ultraviolet (UV) treatment of the Ra-1 cells. Among the survivors of UV-treated Ra-1 cells, a few stable Ra-2 cells were found. When Ra-2 cells were used as the donors, some of them were found to mate via the Ra-1 mode, in analogy with the Ra-1 to Ra-2 alteration with inversion of F mentioned above. Related experiments suggested that the inversion occurs by detachment of the F factor from one Hfr origin locus, followed by reassociation of the F factor with the other Hfr origin locus. Both the Ra-1 and Ra-2 strains reverted spontaneously to an F(+) strain, called RaF(+). Cultures of RaF(+) cells were found to mate primarily according to the Ra-1 and Ra-2 transfer modes, with smaller contributions also coming from transfer modes with origins elsewhere on the chromosome in a way which is similar to the transfer of markers from a normal F(+) strain. The RaF(+) sex factor was found to be wild type, whereas the chromosome was found to carry irregularities (sex factor affinity loci) at the locations of the Ra-1 and Ra-2 origins. Only about 10% of the donor capacity of the RaF(+) strain was due to stable spontaneous Hfr cells in cultures of RaF(+) cells. |
| | |
Authors:
|
B Low |
Related Documents
:
|
20080407 - Introducing the bacterial 'chromid': not a chromosome, not a plasmid. 1911947 - Substitution of umud' for umud does not affect sos mutagenesis. 11943767 - The cytochrome p450 complement (cypome) of streptomyces coelicolor a3(2). 2469627 - Identification of a gene required for the terminal step in erythromycin a biosynthesis ... 8355607 - Electrophoretic karyotyping of the lignin-degrading basidiomycete phanerochaete chrysos... 14990347 - Activity of partially inhibited serine palmitoyltransferase is sufficient for normal sp... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of bacteriology Volume: 93 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1967 Jan |
Date Detail:
|
Created Date: 1967-05-10 Completed Date: 1967-05-10 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 98-106 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromosome Mapping Chromosomes* DNA, Bacterial* Escherichia coli / cytology* Mutation |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Methionine and the regulation of ribonucleic acid synthesis in Escherichia coli.
Next Document: Mutagenesis by near-visible light.