Document Detail


Inversion in Volvox tertius: the effects of con A.
MedLine Citation:
PMID:  7276096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During the development of Volvox tertius spheroids, a single-celled gonidium enlarges and undergoes multiple incomplete cleavages to give an embryo which is 'inside-out' with respect to the adult organism. A morphogenetic movement, termed 'inversion', turns this hollow ball of cells 'inside-out' through a hole, the phialopore. In V. tertius this phialopore possesses 4 inwardly directed lips. Normal inversion was studied in vitro in slide chambers and involved cell-shape changes accompanied by the production of pseudopodia and the bending backwards of the phialopore lips. 100 micrograms/ml Con A specifically and reversibly blocked inversion. Despite the inhibitory effect on cell division, the blocking of inversion was not due to the blocking of the last cell division some 50-100 min prior to inversion. Neither did the first cell-shape change from pear- to spindle-shape appear blocked. A feature of inhibition by Con A was the enhanced production of pseudopodia by embryos blocked at inversion, and the abnormal production of pseudopodia by embryos blocked at earlier stages. Non-inverting embryos showed internal flagella. We suggest that the Con A block to inversion, which may be reversed by alpha-methyl mannoside, arises from the prevention of backwards-bending of the phialopore lips. Fluorescein-isothiocyanate-Con A bound to embryo and cell coat, ane more strongly to the embryo at pre-inversion. SDS-polyacrylamide gel analysis of proteins isolated from embryos showed 4 glycoprotein bands, but Con A binding to these bands could not be demonstrated.
Authors:
G W Ireland; S E Hawkins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  48     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  355-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Algae, Green / cytology*,  drug effects
Cell Division
Concanavalin A / antagonists & inhibitors,  pharmacology*
Methylmannosides / pharmacology
Morphogenesis / drug effects
Time Factors
Chemical
Reg. No./Substance:
0/Methylmannosides; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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