| Inversion of configuration during hydrolysis of alpha-1,4-galacturonidic linkage by three Aspergillus polygalacturonases. | |
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MedLine Citation:
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PMID: 8605979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endopolygalacturonases I and II (PGI and PGII) of Aspergillus niger and an exopolygalacturonase (ExoPG) of A. tubingensis were investigated to reveal the stereochemistry of their hydrolytic action. Reduced pentagalacturonic acid (pentaGalU-ol) and reduced trigalacturonic acid (triGalU-ol) were used as non-reducing substrates for the enzymes. The configuration of the reducing ends in the products formed in D2O reaction mixtures was followed by 1H-NMR spectroscopy. It has been unambiguously established that primary cleavage of pentaGalU-ol by both PGI and PGII leads to diGalU-ol and the beta-anomer of triGalUA. The primary products of hydrolysis of triGalUA-ol by ExoPG were diGal-ol and the beta-anomer of GalUA. Thus, all three Aspergillus polygalacturonases belong to the so-called inverting glycanases, i.e. they utilize the single displacement mechanism of hydrolysis of the glycosidic linkage. |
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Authors:
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P Biely; J Benen; K Heinrichová; H C Kester; J Visser |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 382 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1996-05-17 Completed Date: 1996-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 249-55 Citation Subset: IM |
Affiliation:
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Institute of Chemistry, Slovak Academy of Sciences, Slovak Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus
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enzymology* Aspergillus niger / enzymology* Carbohydrate Conformation Hexuronic Acids / chemistry, metabolism* Hydrolysis Oligosaccharides / metabolism Polygalacturonase / metabolism* Stereoisomerism Sugar Acids / metabolism Trisaccharides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Hexuronic Acids; 0/Oligosaccharides; 0/Sugar Acids; 0/Trisaccharides; 0/pentagalacturonic acid; 14982-50-4/galacturonic acid; 6037-45-2/O-galactopyranosyluronic acid-(1-4)-O-galactopyranosyluronic acid-(1-4)-galactopyranosyluronic acid; EC 3.2.1.15/Polygalacturonase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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