Document Detail

Inversion of configuration during hydrolysis of alpha-1,4-galacturonidic linkage by three Aspergillus polygalacturonases.
MedLine Citation:
PMID:  8605979     Owner:  NLM     Status:  MEDLINE    
Endopolygalacturonases I and II (PGI and PGII) of Aspergillus niger and an exopolygalacturonase (ExoPG) of A. tubingensis were investigated to reveal the stereochemistry of their hydrolytic action. Reduced pentagalacturonic acid (pentaGalU-ol) and reduced trigalacturonic acid (triGalU-ol) were used as non-reducing substrates for the enzymes. The configuration of the reducing ends in the products formed in D2O reaction mixtures was followed by 1H-NMR spectroscopy. It has been unambiguously established that primary cleavage of pentaGalU-ol by both PGI and PGII leads to diGalU-ol and the beta-anomer of triGalUA. The primary products of hydrolysis of triGalUA-ol by ExoPG were diGal-ol and the beta-anomer of GalUA. Thus, all three Aspergillus polygalacturonases belong to the so-called inverting glycanases, i.e. they utilize the single displacement mechanism of hydrolysis of the glycosidic linkage.
P Biely; J Benen; K Heinrichová; H C Kester; J Visser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  382     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-05-17     Completed Date:  1996-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  249-55     Citation Subset:  IM    
Institute of Chemistry, Slovak Academy of Sciences, Slovak Republic.
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MeSH Terms
Aspergillus / enzymology*
Aspergillus niger / enzymology*
Carbohydrate Conformation
Hexuronic Acids / chemistry,  metabolism*
Oligosaccharides / metabolism
Polygalacturonase / metabolism*
Sugar Acids / metabolism
Trisaccharides / metabolism
Reg. No./Substance:
0/Hexuronic Acids; 0/Oligosaccharides; 0/Sugar Acids; 0/Trisaccharides; 0/pentagalacturonic acid; 14982-50-4/galacturonic acid; 6037-45-2/O-galactopyranosyluronic acid-(1-4)-O-galactopyranosyluronic acid-(1-4)-galactopyranosyluronic acid; EC

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