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Inverse relationship between net electric charge on the antigen and that on the sensitized cell in cellular immune response: demonstration with basic encephalitogen of the brain.
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MedLine Citation:
PMID:  51899     Owner:  NLM     Status:  MEDLINE    
An inverse relationship exists between the net-electrical charge of immunogens and the antibodies elicited (1). The cellular basis of the net charge phenomenon has been established for both positively and negatively charged immunogens, by cell separation techniques over columns of opposite charge (7, 8). To establish whether this phenomenon can be extended to include cell-mediated immunity, the response to basic encephalitogenic protein (BE) which induces experimental allergic encephalomyelitis (EAE) was now investigated. Lymph node cells from sensitized strain 13 guinea pigs were fractionated over positively and negatively charged columns and compared to unfractionated cell populations in two assay systems: (a) in vitro response to BE in terms of lymphocyte transformation and (b) the passive transfer of EAE to unsensitized syngeneic recipients. The response was found to be confined to the fraction of cells eluted from glass bead columns, namely, the more negative cells. Cells eluted from poly-L-lysine-coated glass bead columns (i.e., positive cells) were devoid of the capacity to respond to this antigen either in vivo or in vitro. It was previously established that thymocytes rather than bone marrow cells account for the inverse charge phenomenon as assayed by T-helper-cell function in in vivo antibody production (8). We have now extended the inverse charge effect to include cell-mediated immune response of the delayed hypersensitivity type.
D Teitelbaum; C Webb; H Rauch; Y Karniely; R Arnon; M Sela
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  142     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1975 Sep 
Date Detail:
Created Date:  1975-12-11     Completed Date:  1975-12-11     Revised Date:  2010-09-01    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  701-8     Citation Subset:  AIM; IM    
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MeSH Terms
Antigens / analysis*
Chromatography, Affinity
Encephalomyelitis, Autoimmune, Experimental / immunology
Guinea Pigs
Immunity, Cellular*
Immunization, Passive
Lectins / immunology
Lymph Nodes / physiology,  transplantation
Lymphocyte Activation
Muramidase / immunology
Mycobacterium tuberculosis / immunology
Myelin Basic Proteins / analysis,  physiology*
Spinal Cord / transplantation
Reg. No./Substance:
0/Antigens; 0/Lectins; 0/Myelin Basic Proteins; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 9 Year: 1975
Volume: 142 Issue: 3
First Page: 701 Last Page: 708
ID: 2189932
Publisher Id: 76026381
PubMed Id: 51899

Inverse relationship between net electric charge on the antigen and that on the sensitized cell in cellular immune response: demonstration with basic encephalitogen of the brain

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