Document Detail


Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
MedLine Citation:
PMID:  10092453     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have undertaken the inventory and assembly of the ATP binding cassette (ABC) transporter systems in the complete genome of Bacillus subtilis. We combined the identification of the three protein partners that compose an ABC transporter (nucleotide-binding domain, NBD; membrane spanning domain, MSD; and solute-binding protein, SBP) with constraints on the genetic organization. This strategy allowed the identification of 86 NBDs in 78 proteins, 103 MSD proteins and 37 SBPs. The analysis of transcriptional units allows the reconstruction of 59 ABC transporters, which include at least one NBD and one MSD. A particular class of five dimeric ATPases was not associated to MSD partners and is assumed to be involved either in macrolide resistance or regulation of translation elongation. In addition, we have detected five genes encoding ATPases without any gene coding for MSD protein in their neighborhood and 11 operons that encode only the membrane and solute-binding proteins. On the bases of similarities, three ATP-binding proteins are proposed to energize ten incomplete systems, suggesting that one ATPase may be recruited by more than one transporter. Finally, we estimate that the B. subtilis genome encodes for at least 78 ABC transporters that have been split in 38 importers and 40 extruders. The ABC systems have been further classified into 11 sub-families according to the tree obtained from the NBDs and the clustering of the MSDs and the SBPs. Comparisons with Escherichia coli show that the extruders are over-represented in B. subtilis, corresponding to an expansion of the sub-families of antibiotic and drug resistance systems.
Authors:
Y Quentin; G Fichant; F Denizot
Related Documents :
17103013 - Cloning of two sos1 transporters from the seagrass cymodocea nodosa. sos1 transporters ...
21309733 - Presence of task-1 channel in the laryngeal mucosa in the newborn lamb.
12882153 - High-level expression of a soluble snake venom enzyme, gloshedobin, in e. coli in the p...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular biology     Volume:  287     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-06-07     Completed Date:  1999-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  467-84     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie CNRS, 31, Chemin Joseph Aiguier, Marseille Cedex 20, 13402, France. quentin@ir21cb.cnrs-mrs.fr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / classification*,  genetics,  metabolism
Bacillus subtilis / genetics,  metabolism*
Binding Sites / genetics
Evolution, Molecular
Genome, Bacterial
Macromolecular Substances
Membranes / metabolism
Nucleotides / metabolism
Chemical
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Macromolecular Substances; 0/Nucleotides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Lack of evolutionary stasis during alternating replication of an arbovirus in insect and mammalian c...
Next Document:  Differential binding of the Escherichia coli HU, homodimeric forms and heterodimeric form to linear,...