| Invasive differences among Porphyromonas gingivalis strains from healthy and diseased periodontal sites. | |
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MedLine Citation:
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PMID: 18544120 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVE: The purpose of this study was to determine any difference between Porphyromonas gingivalis isolates from periodontally healthy sites as compared to those from diseased sites with respect to the ability to invade host cells. MATERIAL AND METHODS: Subgingival plaque samples were obtained from periodontally healthy and diseased sites using paper points. P. gingivalis colonies were isolated and tested, using an antibiotic protection assay, for their ability to invade KB cells. P. gingivalis 381 and Escherichia coli MC1061 were used as controls. RESULTS: Mean values of 16.79 +/- 0.86 x 10(3) colony-forming units/mL and 26.14 +/- 2.11 x 10(3) colony-forming units/mL were observed in invasion assays for isolates from periodontally healthy and diseased sites, respectively. P. gingivalis present in diseased sites had significantly greater invasive abilities than strains isolated from healthy sites. No statistical difference was noted between male or female subjects concerning the degree of invasion; isolates from diseased sites from both genders had significantly greater invasion abilities than those from healthy sites. A significant correlation was found between the increased invasive capabilities of P. gingivalis isolates vs. an increased probing depth. CONCLUSION: The increased invasion noted with P. gingivalis isolates from diseased sites vs. healthy sites, and the increased invasive capabilities with increasing probing depth, indicate that P. gingivalis isolates have a varying ability to invade host cells in the periodontal pocket. |
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Authors:
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K A Jandik; M Bélanger; S L Low; B R Dorn; M C K Yang; A Progulske-Fox |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-06-09 |
Journal Detail:
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Title: Journal of periodontal research Volume: 43 ISSN: 1600-0765 ISO Abbreviation: J. Periodont. Res. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-10 Completed Date: 2009-01-27 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0055107 Medline TA: J Periodontal Res Country: Denmark |
Other Details:
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Languages: eng Pagination: 524-30 Citation Subset: D; IM |
Affiliation:
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Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Case-Control Studies Chronic Periodontitis / microbiology* Colony Count, Microbial Female Humans KB Cells Linear Models Male Periodontium / microbiology* Porphyromonas gingivalis / pathogenicity* Species Specificity Virulence Factors |
| Grant Support | |
ID/Acronym/Agency:
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DE 013545/DE/NIDCR NIH HHS; DE 07496/DE/NIDCR NIH HHS; R01 DE013545-08/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Virulence Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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