Document Detail


Invasive depth of extravillous trophoblast correlates with cellular phenotype: a comparison of intra- and extrauterine implantation sites.
MedLine Citation:
PMID:  12029487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During intrauterine human placentation, extravillous trophoblast invades uterine tissues starting with proliferating stem cells at the basement membrane of anchoring villi. Transition to the postproliferative invasive phenotype takes place several cell layers distant. Here we show that in intrauterine pregnancies invasive trophoblast comprises three cellular phenotypes: a. Small spindle-shaped trophoblast cells are found along the whole invasive pathway throughout pregnancy. They are embedded in little heterogeneous extracellular matrix but expose only fibronectin receptors (integrins alpha5beta1, alphavbeta3/5), resulting in a partial integrin-matrix mismatch. b. Large polygonal trophoblast cells are rare in early pregnancy but increase in number towards term. They secrete ample heterogeneous extracellular matrix and expose integrins specifically matching the opposing matrix molecules (integrins alpha6beta4, alpha5beta1). c. Multinucleated giant cells in all stages of pregnancy form a kind of peripheral shell of trophoblast. In contrast to intrauterine pregnancies, in viable tubal pregnancies, Mib-1 expression indicating proliferation, extends deeply into the invasive pathway. Trophoblast cells of the invasive pathway mostly belong to the small spindle-shaped phenotype and secrete little extracellular matrix, mainly fibronectins. At the transition to the second cellular layer of cell columns expression of integrin alpha6beta4 switches to expression of alpha5beta1 and alphavbeta3/5. Viable tubal pregnancies are characterised by a broad overlap of proliferative with invasive phenotype as well as a general integrin-matrix mismatch. The differences in proliferation patterns, cellular phenotype and matrix-integrin co-localisation may well explain the increase of invasiveness of normal extravillous trophoblast from term intrauterine via early intrauterine to viable tubal pregnancies.
Authors:
Birgit Kemp; Sonja Kertschanska; Mamed Kadyrov; Werner Rath; Peter Kaufmann; Berthold Huppertz
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2002-04-20
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  117     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-24     Completed Date:  2002-10-03     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  401-14     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, University Hospital RWTH Aachen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Cell Count
Cell Division
Cell Movement*
Embryo Implantation
Extracellular Matrix Proteins / analysis
Female
Humans
Immunohistochemistry
Keratin-7
Keratins / analysis
Microscopy, Electron
Models, Biological
Pregnancy
Pregnancy Trimester, First
Pregnancy, Tubal / pathology
Receptors, Fibronectin / analysis
Trophoblasts / chemistry,  cytology*,  ultrastructure
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/KRT7 protein, human; 0/Keratin-7; 0/Receptors, Fibronectin; 68238-35-7/Keratins

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