Document Detail

Invasion potential and N-acetylgalactosamine expression in a human melanoma model.
MedLine Citation:
PMID:  9466664     Owner:  NLM     Status:  MEDLINE    
Reactivity of the N-acetylgalactosamine-binding Helix pomatia agglutinin (HPA) in tumours has been associated with poor prognosis and metastasis development. In our LOX/FEMX-I human melanoma model, the binding of HPA correlates with experimental lung metastasis formation in athymic nude mice. In the present study, the metastatic potential of 2 human melanoma cell lines (LOX and FEMX-I) was assessed in relation to carbohydrate and invasive phenotype. Immunocytological and invasion assays highlighted significant differences between these 2 cell lines. Immuno-cytochemical analysis confirmed the widespread expression of HPA-binding glycoconjugates on LOX but not FEMX-I cells. One of these HPA-binding glycoconjugates, the Tn antigen, was expressed highly on the surface of LOX cells but only weakly in the cytoplasm of FEMX-I cells. The sialyl Tn antigen was expressed in FEMX-I but not in LOX cells. There was no difference between the cell lines in adhesion/rate of trapping in athymic nude mouse lung tissues. In Matrigel invasion assays, LOX cells demonstrated an invasion potential more than 6 times greater than that observed with FEMX-I cells. Matrigel invasion of LOX cells was inhibited after incubation with HPA (89%) compared to controls with HPA and GalNAc blocking sugar or without HPA (p < 0.0005 at 5 df). In contrast, there was no inhibitory effect with the anti-Tn antibody IE3. Invasion of FEMX-I cells was not affected by the lectin and the IE3 antibody. Immuno-cytochemical analysis revealed expression of the terminal galactose- and polylactosamine-binding lectin galectin 3 (Mac-2) in these melanoma cell lines. Expression of both the lectin and its receptor may be a contributory feature in the pulmonary invasion of LOX melanoma cells. Overall, our findings suggest that HPA-binding glycoconjugates other than the alphaGalNAc-O-Ser/Thr of the Tn antigen may be important in the extracellular matrix invasion of LOX melanoma cells.
P D Rye; O Fodstad; E Emilsen; M Bryne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  75     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-02-26     Completed Date:  1998-02-26     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  609-14     Citation Subset:  IM    
Department of Tumour Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo.
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MeSH Terms
Acetylgalactosamine / metabolism*
Antigens, Differentiation / physiology
Antigens, Tumor-Associated, Carbohydrate / metabolism
Drug Combinations
Galectin 3
Glycoconjugates / metabolism
Lung Neoplasms / pathology,  secondary
Melanoma / immunology,  metabolism,  pathology*
Mice, Inbred BALB C
Mice, Nude
Neoplasm Invasiveness
Neoplasm Metastasis*
Neoplasm Transplantation
Transplantation, Heterologous
Reg. No./Substance:
0/Antigens, Differentiation; 0/Antigens, Tumor-Associated, Carbohydrate; 0/Drug Combinations; 0/Galectin 3; 0/Glycoconjugates; 0/Laminin; 0/Proteoglycans; 0/Tn antigen; 119978-18-6/matrigel; 31022-50-1/Acetylgalactosamine; 9007-34-5/Collagen

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