Document Detail


Invasion of human coronary artery cells by periodontal pathogens.
MedLine Citation:
PMID:  10531230     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is an emerging paradigm shift from coronary heart disease having a purely hereditary and nutritional causation to possibly having an infectious etiology. Recent epidemiological studies have shown a correlation between periodontal disease and coronary heart disease. However, to date, there is minimal information as to the possible disease mechanisms of this association. It is our hypothesis that invasion of the coronary artery cells by oral bacteria may start and/or exacerbate the inflammatory response in atherosclerosis. Since a few periodontal pathogens have been reported to invade oral epithelial tissues, we tested the ability of three putative periodontal pathogens-Eikenella corrodens, Porphyromonas gingivalis, and Prevotella intermedia-to invade human coronary artery endothelial cells and coronary artery smooth muscle cells. In this study we demonstrate by an antibiotic protection assay and electron microscopy that specific species and strains invade coronary artery cells at a significant level. Actin polymerization and eukaryotic protein synthesis in metabolically active cells were required since the corresponding inhibitors nearly abrogated invasion. Many intracellular P. gingivalis organisms were seen to be present in multimembranous vacuoles resembling autophagosomes by morphological analysis. This is the first report of oral microorganisms invading human primary cell cultures of the vasculature.
Authors:
B R Dorn; W A Dunn; A Progulske-Fox
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  67     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-16     Completed Date:  1999-11-16     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5792-8     Citation Subset:  IM    
Affiliation:
Department of Oral Biology, College of Dentistry, College of Medicine, University of Florida, Gainesville, Florida 32610, USA.
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MeSH Terms
Descriptor/Qualifier:
Coronary Vessels / microbiology*,  ultrastructure
Cytochalasin D / pharmacology
Eikenella corrodens / physiology*
Endothelium, Vascular / microbiology,  ultrastructure
Humans
KB Cells
Microscopy, Electron
Porphyromonas gingivalis / physiology*
Prevotella intermedia / physiology*
Temperature
Grant Support
ID/Acronym/Agency:
DE 07496/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
22144-77-0/Cytochalasin D
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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