Document Detail


Invasion and biofilm formation of Burkholderia dolosa is comparable with Burkholderia cenocepacia and Burkholderia multivorans.
MedLine Citation:
PMID:  16781896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Colonisation with Burkholderia cepacia complex pathogens has been associated with accelerated decline in cystic fibrosis (CF) patients. The two most common species among the CF community are Burkholderia cenocepacia and Burkholderia multivorans. However, Burkholderia dolosa has recently been causing concern due to its transmissibility and virulence in CF patients. METHODS: We have compared the ability of five B. dolosa strains to invade lung epithelial cells in vitro with other members of the Bcc. The bacterial epithelial cell interaction was visualised by transmission electron microscopy. We have also examined the ability of these strains to form biofilms in vitro. RESULTS: We have found that members of this species can invade pulmonary epithelial cells in vitro as readily as those from B. cenocepacia and B. multivorans. Confirmation of intracellular invasion was obtained by transmission electron microscopy. B. dolosa strains were readily observed in membrane bound vesicles inside the lung epithelial cells. In addition, strains from this species were capable of forming strong biofilms at a level comparable to the more clinically relevant species. CONCLUSIONS: B. dolosa shows comparable virulence characteristics in vitro to the two most clinically relevant species indicating precautions should be taken when this species is identified in the CF population.
Authors:
Emma Caraher; Caroline Duff; Tracy Mullen; Suzanne Mc Keon; Philip Murphy; Máire Callaghan; Siobhán McClean
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Publication Detail:
Type:  Journal Article     Date:  2006-06-15
Journal Detail:
Title:  Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society     Volume:  6     ISSN:  1569-1993     ISO Abbreviation:  J. Cyst. Fibros.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-09     Completed Date:  2007-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128966     Medline TA:  J Cyst Fibros     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  49-56     Citation Subset:  IM    
Affiliation:
Department of Applied Science, National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Biofilms / growth & development*
Burkholderia Infections
Burkholderia cepacia complex / growth & development*,  pathogenicity
Cell Line
Cystic Fibrosis / microbiology
Cytoplasmic Vesicles / microbiology*
Humans
Microscopy, Electron, Transmission
Respiratory Mucosa / microbiology
Virulence

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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