Document Detail


Invasion of Burkholderia cepacia complex isolates into lung epithelial cells involves glycolipid receptors.
MedLine Citation:
PMID:  20826205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Burkholderia cepacia complex (Bcc) is a group of opportunistic cystic fibrosis (CF) pathogens that invade lung epithelial cells. The mechanisms of invasion are poorly understood, in particular, the receptors utilised by this bacterium in the invasion process have not been identified. The aim of this study was to investigate the epithelial receptors involved in the invasion of Bcc isolates. We confirmed that invasion into two lung epithelial cell lines (16HBE14o- and CFBE41o-) which have a non-CF and CF phenotype, respectively, is receptor mediated and showed that pre-treatment of these epithelial cell lines with α- or β-galactosidase reduced invasion of isolates of two species of Bcc, Burkholderia multivorans and Burkholderia cenocepacia. In contrast, removal of mucin had no significant effect. Biotinylated Bcc strains were shown to bind to purified glycolipids separated by thin layer chromatography, albeit different patterns of binding were associated with different strains. Invasion of CF lung epithelial cells (CFBE41o-) by all three Bcc strains examined was significantly reduced by treatment of cells with inhibitors of glycolipid biosynthesis. Although the specific glycolipid involved in each case has not been elucidated, it is apparent that invasion of lung epithelial cells is mediated via binding to glycosphingolipid receptors.
Authors:
Tracy Mullen; Máire Callaghan; Siobhán McClean
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-06
Journal Detail:
Title:  Microbial pathogenesis     Volume:  49     ISSN:  1096-1208     ISO Abbreviation:  Microb. Pathog.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  England    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Centre of Microbial Host Interactions, Institute of Technology, Tallaght, Dublin 24, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion*
Burkholderia cepacia complex / pathogenicity*
Cell Line
Epithelial Cells / microbiology*
Glycosphingolipids / metabolism*
Humans
Receptors, Cell Surface / metabolism*
alpha-Galactosidase / metabolism
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/Glycosphingolipids; 0/Receptors, Cell Surface; 0/glycolipid receptor; EC 3.2.1.22/alpha-Galactosidase; EC 3.2.1.23/beta-Galactosidase

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