Document Detail

Intron 1 rather than 5' flanking sequence mediates cell type-specific expression of c-myb at level of transcription elongation.
MedLine Citation:
PMID:  10786619     Owner:  NLM     Status:  MEDLINE    
Previous studies have shown that expression of steady-state c-myb mRNA was regulated primarily by a block in intron 1 during transcription elongation. This study shows that the block site maps approximately 1700 bp from the start of the intron. Studies based on a reporter construct containing c-myb flanking region and intron 1 suggest that the flanking region is not important in the regulation of the cell type-specific expression of c-myb. RNA splicing of intron 1 may enhance the expression in a non-cell type-specific manner. A conserved intron domain comprising the block site is required for defining this site, but this function of the domain is independent of cell type. The cell type-specific regulation of c-myb transcription elongation is mediated by a 5' intron sequence. A mechanism for down regulation of c-myb gene expression by the block to transcription elongation has been proposed.
W Yuan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1490     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-05-24     Completed Date:  2000-05-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  74-86     Citation Subset:  IM    
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
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MeSH Terms
3T3 Cells
Chloramphenicol O-Acetyltransferase / analysis
Gene Expression Regulation*
Genes, myb*
Terminal Repeat Sequences
Transcription, Genetic*
Reg. No./Substance:
EC O-Acetyltransferase

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