| Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization. | |
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MedLine Citation:
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PMID: 21956131 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Swertia tetraptera Maxim. is an important source of secoiridoid glucosides. To produce these pharmacologically valuable compounds heterologously in somatic hybrid cell lines, S. tetraptera protoplasts were irradiated with various doses of UV light and fused with protoplasts from a long-term cell line of Bupleurum scorzonerifolium Willd. This recipient was chosen as the cell line is cytogenetically stable and fast growing; furthermore, protoplasts isolated from the cell line are readily regenerable. From a set of 86 putative hybrid calli, only two were able to regenerate viable green plants. The hybridity of the 19 of the 86 selections was revealed by a combined isozyme and RAPD analysis, supported by a karyotypic study based on genomic in situ hybridization (GISH). Clone I-3 contained 0.014% swertiamarin while the regenerants had 0.069% swertiamarin and 0.409% gentiopicroside while the III-4 plants contained only 0.015% gentiopicroside. |
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Authors:
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Li Jiang; Yunfei Cai; Guangmin Xia; Fengning Xiang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-09-29 |
Journal Detail:
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Title: Protoplasma Volume: 249 ISSN: 1615-6102 ISO Abbreviation: Protoplasma Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-25 Completed Date: 2012-10-17 Revised Date: 2013-01-24 |
Medline Journal Info:
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Nlm Unique ID: 9806853 Medline TA: Protoplasma Country: Austria |
Other Details:
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Languages: eng Pagination: 737-45 Citation Subset: IM |
Affiliation:
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Anhui Medical University, Hefei, 230061, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bupleurum
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cytology,
genetics*,
metabolism Chromatography, High Pressure Liquid Chromosomes, Plant / metabolism Culture Techniques Esterases / genetics Gene Flow Genome, Chloroplast Genotype Hybridization, Genetic* Iridoid Glucosides / isolation & purification, metabolism Isoenzymes / genetics Karyotype Microsatellite Repeats Plant Proteins / genetics Protoplasts / metabolism Pyrones / isolation & purification, metabolism Swertia / cytology, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Iridoid Glucosides; 0/Isoenzymes; 0/Plant Proteins; 0/Pyrones; 0WE09Z21RC/gentiopicroside; 17388-39-5/swertiamarin; EC 3.1.-/Esterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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