Document Detail


Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization.
MedLine Citation:
PMID:  21956131     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Swertia tetraptera Maxim. is an important source of secoiridoid glucosides. To produce these pharmacologically valuable compounds heterologously in somatic hybrid cell lines, S. tetraptera protoplasts were irradiated with various doses of UV light and fused with protoplasts from a long-term cell line of Bupleurum scorzonerifolium Willd. This recipient was chosen as the cell line is cytogenetically stable and fast growing; furthermore, protoplasts isolated from the cell line are readily regenerable. From a set of 86 putative hybrid calli, only two were able to regenerate viable green plants. The hybridity of the 19 of the 86 selections was revealed by a combined isozyme and RAPD analysis, supported by a karyotypic study based on genomic in situ hybridization (GISH). Clone I-3 contained 0.014% swertiamarin while the regenerants had 0.069% swertiamarin and 0.409% gentiopicroside while the III-4 plants contained only 0.015% gentiopicroside.
Authors:
Li Jiang; Yunfei Cai; Guangmin Xia; Fengning Xiang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-29
Journal Detail:
Title:  Protoplasma     Volume:  249     ISSN:  1615-6102     ISO Abbreviation:  Protoplasma     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-25     Completed Date:  2012-10-17     Revised Date:  2013-01-24    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  737-45     Citation Subset:  IM    
Affiliation:
Anhui Medical University, Hefei, 230061, China.
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MeSH Terms
Descriptor/Qualifier:
Bupleurum / cytology,  genetics*,  metabolism
Chromatography, High Pressure Liquid
Chromosomes, Plant / metabolism
Culture Techniques
Esterases / genetics
Gene Flow
Genome, Chloroplast
Genotype
Hybridization, Genetic*
Iridoid Glucosides / isolation & purification,  metabolism
Isoenzymes / genetics
Karyotype
Microsatellite Repeats
Plant Proteins / genetics
Protoplasts / metabolism
Pyrones / isolation & purification,  metabolism
Swertia / cytology,  genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Iridoid Glucosides; 0/Isoenzymes; 0/Plant Proteins; 0/Pyrones; 0WE09Z21RC/gentiopicroside; 17388-39-5/swertiamarin; EC 3.1.-/Esterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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