Document Detail


Introgression of a disrupted cadherin gene enables susceptible Helicoverpa armigera to obtain resistance to Bacillus thuringiensis toxin Cry1Ac.
MedLine Citation:
PMID:  18954492     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A disrupted allele (r1) of a cadherin gene (Ha_BtR) is genetically associated with incompletely recessive resistance to Bacillus thuringiensis toxin Cry1Ac in a Cry1Ac-selected strain (GYBT) of Helicoverpa armigera. The r1 allele of Ha_BtR was introgressed into a susceptible SCD strain by crossing the GYBT strain to the SCD strain, followed by repeated backcrossing to the SCD strain and molecular marker assisted family selection. The introgressed strain (designated as SCD-r1, carrying homozygous r1 allele) obtained 438-fold resistance to Cry1Ac, >41-fold resistance to Cry1Aa and 31-fold resistance Cry1Ab compared with the SCD strain; however, there was no significant difference in susceptibility to Cry2Aa between the integrated and parent strains. It confirms that the loss of function mutation of Ha_BtR alone can confer medium to high levels of resistance to the three Cry1A toxins in H. armigera. Reciprocal crosses between the SCD and SCD-r1 strains showed that resistance to Cry1Ac in the SCD-r1 strain was completely recessive. Life tables of the SCD and SCD-r1 strains on artificial diet in the laboratory were constructed, and results showed that the net replacement rate (R0) did not differ between the strains. The toxicity of two chemical insecticides, fenvalerate and monocrotophos, against the SCD-r1 strain was not significantly different from that to the SCD strain. However, larval development time of the SCD-r1 strain was significantly longer than that of the SCD strain, indicating a fitness cost of slower larval growth is associated with Ha_BtR disruption in H. armigera.
Authors:
Y-H Yang; Y-J Yang; W-Y Gao; J-J Guo; Y-H Wu; Y-D Wu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-28
Journal Detail:
Title:  Bulletin of entomological research     Volume:  99     ISSN:  1475-2670     ISO Abbreviation:  Bull. Entomol. Res.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-09     Completed Date:  2009-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984715R     Medline TA:  Bull Entomol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  175-81     Citation Subset:  IM    
Affiliation:
Key Lab of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Bacterial Proteins / toxicity*
Bacterial Toxins / toxicity*
Cadherins / genetics*
Drug Resistance / genetics*
Endotoxins / toxicity*
Female
Hemolysin Proteins / toxicity*
Male
Monocrotophos / toxicity
Moths / drug effects*,  genetics*,  growth & development
Nitriles / toxicity
Pyrethrins / toxicity
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Cadherins; 0/Endotoxins; 0/Hemolysin Proteins; 0/Nitriles; 0/Pyrethrins; 0/insecticidal crystal protein, Bacillus Thuringiensis; 51630-58-1/fenvalerate; 6923-22-4/Monocrotophos

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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