Document Detail


Introduction to beetle luciferases and their applications.
MedLine Citation:
PMID:  2678917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
All beetle luciferases have evolved from a common ancestor: they all use ATP, O2, and a common luciferin as substrates. The most studied of these luciferases is that derived from the firefly Photinus pyralis, a beetle in the superfamily of Cantharoidea. The sensitivity with which the activity of this enzyme can be assayed has made it useful in the measurement of minute concentrations of ATP. With the cloning of the cDNA coding this luciferase, it has also found wide application in molecular biology as a reporter gene. We have recently cloned other cDNAs that code for luciferases from the bioluminescent click beetle, Pyrophorus plagiophthalamus, in the superfamily Elateroidea. These newly acquired luciferases are of at least four different types, distinguishable by their ability to emit different colours of bioluminescence ranging from green to orange. Unique properties of these luciferases, especially their emission of multiple colours, may make them additionally useful in applications.
Authors:
K V Wood; Y A Lam; W D McElroy
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Publication Detail:
Type:  Comparative Study; Journal Article; Review    
Journal Detail:
Title:  Journal of bioluminescence and chemiluminescence     Volume:  4     ISSN:  0884-3996     ISO Abbreviation:  J. Biolumin. Chemilumin.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-11-06     Completed Date:  1989-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8612490     Medline TA:  J Biolumin Chemilumin     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  289-301     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of California, San Diego, La Jolla 92093.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Beetles / enzymology*,  genetics
Cloning, Molecular
DNA / genetics
Luciferases / genetics,  metabolism*
Luminescent Measurements
Molecular Sequence Data
Sequence Homology, Nucleic Acid
Species Specificity
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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