| Introduction of a heterologous nucleus into enucleated cytoplasms of cultured mouse L-cells. | |
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MedLine Citation:
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PMID: 5274477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mouse L-cells exposed to cytochalasin B undergo random nuclear protrusion and, occasionally, total enucleation. The anucleate cell remains viable for several days. Chick erythrocytes were fused to L-cells with inactivated Sendai virus. The resulting hybrids, containing one or more erythrocyte nuclei in addition to the L-cell nucleus, were treated with cytochalasin B. Through the process of random enucleation, the L-cell nucleus was extruded from some hybrid cells while the erythrocyte nucleus was retained in the hybrid. In other experiments, chick erythrocytes were fused directly to enucleated L-cells. The result in both instances is the introduction of an erythrocyte nucleus into L-cell cytoplasm. The erythrocyte nucleus swells and the hybrid cell is capable of incorporating radioactive uridine. |
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Authors:
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R L Ladda; R D Estensen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 67 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1970 Nov |
Date Detail:
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Created Date: 1971-02-02 Completed Date: 1971-02-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1528-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chick Embryo Erythrocytes Hybridization, Genetic* L Cells (Cell Line)* Mice Mycotoxins / pharmacology Nuclear Transfer Techniques* Transplantation, Heterologous* Tritium Uridine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Mycotoxins; 10028-17-8/Tritium; 58-96-8/Uridine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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