| Introduction of phospholipids to cultured cells with cyclodextrin. | |
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MedLine Citation:
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PMID: 20881052 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies indicate that methyl-β-cyclodextrin (meβ-CD) can greatly enhance translocation of long-chain phospholipids from vesicles to cells in culture, which is very useful when studying, e.g., phospholipid metabolism and trafficking. However, the parameters affecting the transfer have not been systematically studied. Therefore, we studied the relevant parameters including meβ-CD and vesicle concentration, incubation time, phospholipid structure, and cell type. Because meβ-CD can extract cholesterol and other lipids from cells, thereby potentially altering cell growth or viability, these issues were studied as well. The results show that efficient incorporation of phospholipid species with hydrophobicity similar to that of natural species can be obtained without significantly compromising cell growth or viability. Cellular content of phosphatidyl-serine, -ethanolamine, and -choline could be increased dramatically, i.e., 400, 125, and 25%, respectively. Depletion of cellular cholesterol could be prevented or alleviated by inclusion of the proper amount of cholesterol in the donor vesicles. In summary, meβ-CD mediates efficient transfer of long-chain (phospho) lipids from vesicles to cells without significantly compromising their growth or viability. This lays a basis for detailed studies of phospholipid metabolism and trafficking as well as enables extensive manipulation of cellular phospholipid composition, which is particularly useful when investigating mechanisms underlying phospholipid homeostasis. |
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Authors:
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Ville Kainu; Martin Hermansson; Pentti Somerharju |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-29 |
Journal Detail:
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Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-10 Completed Date: 2011-02-22 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 3533-41 Citation Subset: IM |
Affiliation:
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Institute of Biomedicine, Department of Medical Biochemistry and Developmental Biology, University of Helsinki, Helsinki, Finland. juho.kainu@helsinki.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Proliferation / drug effects* Cell Survival / drug effects Cells, Cultured Cholesterol / metabolism Cricetinae Cyclodextrins / pharmacology* Fibroblasts / cytology, metabolism HeLa Cells / cytology, metabolism Homeostasis / physiology Humans Hydrophobic and Hydrophilic Interactions Kidney / cytology, embryology, metabolism Liposomes / metabolism* Phospholipids / metabolism* Spectrometry, Mass, Electrospray Ionization Time Factors beta-Cyclodextrins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cyclodextrins; 0/Liposomes; 0/Phospholipids; 0/beta-Cyclodextrins; 0/methyl-beta-cyclodextrin; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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