Document Detail


Introducing human population biology through an easy laboratory exercise on mitochondrial DNA.
MedLine Citation:
PMID:  21567805     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This article describes an easy and cheap laboratory exercise for students to discover their own mitochondrial haplogroup. Students use buccal swabs to obtain mucosa cells as noninvasive tissue samples, extract DNA, and with a simple polymerase chain reaction-restriction fragment length polymorphism analysis they can obtain DNA fragments of different sizes that can be visualized in agarose gels. The analysis of these fragments can reveal the mitochondrial haplogroup of each student. The results of the exercise can be used to provide additional insights into the genetic variation of human populations.
Authors:
Antonio F Pardiñas; Eduardo Dopico; Agustín Roca; Eva Garcia-Vazquez; Belen Lopez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology     Volume:  38     ISSN:  1539-3429     ISO Abbreviation:  Biochem Mol Biol Educ     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2011-05-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100970605     Medline TA:  Biochem Mol Biol Educ     Country:  United States    
Other Details:
Languages:  eng     Pagination:  110-5     Citation Subset:  -    
Copyright Information:
Copyright © 2010 International Union of Biochemistry and Molecular Biology, Inc.
Affiliation:
Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, Oviedo, Spain.
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