| Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast. | |
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MedLine Citation:
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PMID: 18480404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Centrosome duplication must be tightly controlled so that duplication occurs only once each cell cycle. Accumulation of multiple centrosomes can result in the assembly of a multipolar spindle and lead to chromosome mis-segregation and genomic instability. In metazoans, a centrosome-intrinsic mechanism prevents reduplication until centriole disengagement. Mitotic cyclin/cyclin-dependent kinases (CDKs) prevent reduplication of the budding yeast centrosome, called a spindle pole body (SPB), in late S-phase and G2/M, but the mechanism remains unclear. How SPB reduplication is prevented early in the cell cycle is also not understood. Here we show that, similar to metazoans, an SPB-intrinsic mechanism prevents reduplication early in the cell cycle. We also show that mitotic cyclins can inhibit SPB duplication when expressed before satellite assembly in early G1, but not later in G1, after the satellite had assembled. Moreover, electron microscopy revealed that SPBs do not assemble a satellite in cells expressing Clb2 in early G1. Finally, we demonstrate that Clb2 must localize to the cytoplasm in order to inhibit SPB duplication, suggesting the possibility for direct CDK inhibition of satellite components. These two mechanisms, intrinsic and extrinsic control by CDK, evoke two-step system that prevents SPB reduplication throughout the cell cycle. |
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Authors:
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Laura A Simmons Kovacs; Christine L Nelson; Steven B Haase |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-05-14 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 19 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-29 Completed Date: 2008-12-22 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 3243-53 Citation Subset: IM |
Affiliation:
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Department of Biology, Duke University, Durham, NC 27708-0338, USa. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Cell Proliferation Centrosome / metabolism Cyclin B / metabolism Cyclin-Dependent Kinases / metabolism* Cytoplasm / metabolism Gene Expression Regulation, Fungal* Genome Microscopy, Electron Mitosis Mitotic Spindle Apparatus / metabolism Models, Biological Plasmids / metabolism Saccharomyces cerevisiae Proteins / metabolism Saccharomycetales / physiology* |
| Chemical | |
Reg. No./Substance:
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0/CLB2 protein, S cerevisiae; 0/Cyclin B; 0/Saccharomyces cerevisiae Proteins; EC 2.7.11.22/Cyclin-Dependent Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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