Document Detail


Intrinsic apoptosis and NF-κB signaling are potential molecular targets for chemoprevention by black tea polyphenols in HepG2 cells in vitro and in a rat hepatocarcinogenesis model in vivo.
MedLine Citation:
PMID:  20828598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antiproliferative and apoptosis inducing effects of black tea polyphenols (Polyphenon-B) on HepG2 cells in vitro and in a rat hepatocarcinogenesis model in vivo were investigated. Viability of HepG2 cells was evaluated by the MTT assay, and apoptosis by AO-EB and DAPI staining, cell cycle analysis, and annexin V-PI assay. For the in vivo study, male Sprague-Dawley rats treated with dimethylaminoazobenzene (DAB) (0.06%) were used. The expression of Bcl-2 and NF-κB family members were analyzed by immunoblotting. Administration of Polyphenon-B induced dose-dependent inhibition of growth of HepG2 cells and reduced tumor incidence in DAB administered animals. HepG2 cells also exhibited morphological features characteristic of apoptotic cell death. In addition, administration of Polyphenon-B increased the expression of Bax, tBid, Smac/Diablo, cytochrome C, Apaf-1, caspases, and IκB with PARP cleavage, and decreased the expression of Bcl-2, Bcl-xL, pBad, NF-κB, p-IκB-α, IKKβ and Ub in both HepG2 cells and in DAB-treated animals. These results provide evidence that Polyphenon-B effectively inhibits proliferation and induces apoptosis both in vitro and in vivo by inhibiting NF-κB, and inducing intrinsic apoptosis by modulating the expression of a network of interrelated molecules eventually culminating in caspase-mediated cell death.
Authors:
R Senthil Murugan; R Vidya Priyadarsini; K Ramalingam; Y Hara; D Karunagaran; S Nagini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-07
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  48     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-11     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3281-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Carcinogens / toxicity
Carcinoma, Hepatocellular / chemically induced,  pathology,  prevention & control*
Cell Survival / drug effects
Hep G2 Cells
Humans
Liver Neoplasms, Experimental / chemically induced,  pathology,  prevention & control*
Male
NF-kappa B / metabolism*
Phenols / pharmacology*
Plant Extracts / pharmacology
Rats
Rats, Sprague-Dawley
Signal Transduction / drug effects
Tea / chemistry*
p-Dimethylaminoazobenzene / toxicity
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Carcinogens; 0/NF-kappa B; 0/Phenols; 0/Plant Extracts; 0/Tea; 0/polyphenon B; 60-11-7/p-Dimethylaminoazobenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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