Document Detail


Intravital microscopy to image membrane trafficking in live rats.
MedLine Citation:
PMID:  23027003     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. Here, we describe a series of procedures designed to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.
Authors:
Andrius Masedunskas; Monika Sramkova; Laura Parente; Roberto Weigert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  931     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-02-11     Revised Date:  2014-06-12    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-67     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Endocytosis*
Exocytosis
Fluorescent Dyes / chemistry
Gene Transfer Techniques
Microscopy, Confocal
Rats
Rats, Sprague-Dawley
Restraint, Physical / instrumentation
Staining and Labeling
Submandibular Gland / cytology*
Time-Lapse Imaging
Grant Support
ID/Acronym/Agency:
ZIA DE000717-07/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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