Document Detail


Intraventricular hemorrhage induces deposition of proteoglycans in premature rabbits, but their in vivo degradation with chondroitinase does not restore myelination, ventricle size and neurological recovery.
MedLine Citation:
PMID:  23474192     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intraventricular hemorrhage (IVH) results in white matter injury and hydrocephalus in premature infants. Chondroitin sulfate proteoglycans (CSPGs)-neuorcan, brevican, versican, aggrecan and phosphacan-are unregulated in the extracellular matrix after brain injury, and their degradation enhances plasticity of the brain. Therefore, we hypothesized that CSPG levels were elevated in the forebrain of premature infants with IVH and that in vivo degradation of CSPGs would enhance maturation of oligodendrocyte, augment myelination, promote neurological recovery, and minimize hydrocephalus. We found that levels of neurocan, brevican, aggrecan, phosphacan, and versican were elevated, whereas NG2 expression was reduced in premature rabbit pups and human infants with IVH compared to controls. Intracerebroventricular chondroitinase ABC (ChABC) reduced the expression of neuorcan, brevican, versican and aggrecan, but not NG2. However, ChABC treatment did not enhance maturation of oligodendrocytes, myelination, or neurological recovery in the pups with IVH. Moreover, ChABC did not reduce gliosis or ventriculomegaly. Our results demonstrate that IVH induces distinct changes in the components of CSPGs, and that reversing these changes by in vivo ChABC treatment neither promotes clinical recovery, myelination, nor reduces ventriculomegaly in preterm rabbit pups.
Authors:
Govindaiah Vinukonda; Muhammad T Zia; Bala B R Bhimavarapu; Furong Hu; Michelle Feinberg; Aqiba Bokhari; Zoltan Ungvari; Victor A Fried; Praveen Ballabh
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-06
Journal Detail:
Title:  Experimental neurology     Volume:  247     ISSN:  1090-2430     ISO Abbreviation:  Exp. Neurol.     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-08-12     Completed Date:  2013-11-26     Revised Date:  2014-09-02    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  630-44     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Animals, Newborn
Antigens / genetics,  metabolism
Cell Proliferation / drug effects
Cerebral Hemorrhage* / metabolism,  pathology,  physiopathology
Chondroitin ABC Lyase / administration & dosage
Chondroitin Sulfate Proteoglycans / genetics,  metabolism*
Disease Models, Animal
Female
Fetus
Gene Expression Regulation, Developmental / physiology*
Gestational Age
Humans
Infant, Newborn
Male
Motor Activity / drug effects,  physiology
Myelin Sheath / metabolism
Nerve Tissue Proteins / metabolism
Oligodendroglia / metabolism
Pregnancy
Proteoglycans / genetics,  metabolism
Rabbits
Recovery of Function / drug effects,  physiology*
Time Factors
Grant Support
ID/Acronym/Agency:
1R21HD061778-01/HD/NICHD NIH HHS; R01 AT006526/AT/NCCAM NIH HHS; R01 NS071263/NS/NINDS NIH HHS; R01 NS071263/NS/NINDS NIH HHS; R21 HD061778/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antigens; 0/Chondroitin Sulfate Proteoglycans; 0/Nerve Tissue Proteins; 0/Proteoglycans; 0/chondroitin sulfate proteoglycan 4; EC 4.2.2.20/Chondroitin ABC Lyase
Comments/Corrections

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