| Intravenously injected [1-14C]arachidonic acid targets phospholipids, and [1-14C]palmitic acid targets neutral lipids in hearts of awake rats. | |
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MedLine Citation:
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PMID: 10984112 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The differential uptake and targeting of intravenously infused [1-14C]palmitic ([1-14C]16:0) and [1-14C]arachidonic ([1-14C]20:4n-6) acids into heart lipid pools were determined in awake adult male rats. The fatty acid tracers were infused (170 microCi/kg) through the femoral vein at a constant rate of 0.4 mL/min over 5 min. At 10 min postinfusion, the rats were killed using pentobarbital. The hearts were rapidly removed, washed free of exogenous blood, and frozen in dry ice. Arterial blood was withdrawn over the course of the experiment to determine plasma radiotracer levels. Lipids were extracted from heart tissue using a two-phase system, and total radioactivity was measured in the nonvolatile aqueous and organic fractions. Both fatty acid tracers had similar plasma curves, but were differentially distributed into heart lipid compartments. The extent of [1-14C]20:4n-6 esterification into heart phospholipids, primarily choline glycerophospholipids, was elevated 3.5-fold compared to [1-14C]16:0. The unilateral incorporation coefficient, k*, which represents tissue radioactivity divided by the integrated plasma radioactivity for heart phospholipid, was sevenfold greater for [1-14C]20:4n-6 than for [1-14C]16:0. In contrast, [1-14C]16:0 was esterified mainly into heart neutral lipids, primarily triacylglycerols (TG), and was also found in the nonvolatile aqueous compartment. Thus, in rat heart, [1-14C]20:4n-6 was primarily targeted for esterification into phospholipids, while [1-14C]16:0 was targeted for esterification into TG or metabolized into nonvolatile aqueous components. |
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Authors:
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E J Murphy; T A Rosenberger; C B Patrick; S I Rapoport |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 35 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-12-11 Completed Date: 2001-03-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 891-8 Citation Subset: IM |
Affiliation:
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Section on Brain Physiology and Metabolism, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892-1582, USA. murphye@mail.nih.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / pharmacokinetics* Carbon Radioisotopes Chromatography, Thin Layer Femoral Vein / metabolism Injections, Intravenous Lipids / analysis Male Myocardium / metabolism* Oxygen / metabolism Palmitic Acid / pharmacokinetics* Phospholipids / metabolism* Rats Rats, Sprague-Dawley Time Factors Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Lipids; 0/Phospholipids; 0/Triglycerides; 506-32-1/Arachidonic Acid; 57-10-3/Palmitic Acid; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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