Document Detail


Intravenous lipopolysaccharide-induced pulmonary maturation and structural changes in fetal sheep.
MedLine Citation:
PMID:  19110233     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Antenatal pulmonary inflammation is associated with reduced risk for respiratory distress syndrome but with an increased risk for bronchopulmonary dysplasia (BPD) with impaired alveogenesis. OBJECTIVE: We hypothesized that fetal systemic inflammation induced by intravenous (IV) lipopolysaccharide (LPS) would affect lung development in utero. STUDY DESIGN: Twenty-one fetal sheep were instrumented (107 days gestational age). Control fetuses received saline (N = 12) and 9 in the study group received 100 ng of LPS IV 3 days after surgery. Animals were assessed for lung maturation and structure after 3 (N = 5) and 7 (N = 4) days. RESULTS: Interleukin-6 concentration increased in the bronchoalveolar lavage more than 40-fold 3 days after LPS IV. Processing of pro-surfactant protein (SP)-B to mature SP-B and increased SP-B concentrations were shown 7 days after LPS IV. Deposition of elastin fibers at sites of septation was disturbed within 3 days after LPS IV. CONCLUSION: Lung maturation and disturbed lung structure occurred after short-term exposure to fetal inflammation and suggests new targeted therapies for BPD.
Authors:
Boris W Kramer; Andreas Ladenburger; Steffen Kunzmann; Christian P Speer; Jasper V Been; J Freek van Iwaarden; Luc J I Zimmermann; Markus Gantert; Yves Garnier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-25
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  200     ISSN:  1097-6868     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  195.e1-10     Citation Subset:  AIM; IM    
Affiliation:
Department of Pediatrics, Maastricht University Hospital Center, the Netherlands. bkra@paed.azm.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation
Elastin / immunology
Female
Fetal Diseases / immunology*
Fetal Organ Maturity / immunology*
Fetus
Lipopolysaccharides / administration & dosage*
Lung / growth & development,  immunology*
Lung Compliance
Lung Diseases / immunology*
Pulmonary Surfactant-Associated Protein B / immunology
Sheep
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 0/Pulmonary Surfactant-Associated Protein B; 9007-58-3/Elastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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