| Intravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. | |
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MedLine Citation:
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PMID: 15967439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intravenous infusion of bone marrow cells has demonstrated therapeutic efficacy in animal models of cerebral ischemia and spinal cord injury. We intravenously delivered human mesenchymal stem cells (SH2+, SH3+, CD34-, and CD45-) immortalized with a human-telomerase gene (hTERT-MSCs) and transfected with eGFP or LacZ into rats 12 h after induction of transient middle cerebral artery occlusion (MCAO), to study their potential therapeutic benefit. hTERT-MSCs were delivered at 12 h after lesion induction. Lesion size was assessed using MR imaging and spectroscopy, and histological methods. Functional outcome was assessed using the Morris water maze and a treadmill test. Intravenous delivery of hTERT-MSCs reduced lesion volume and the magnitude of the reduction and functional improvement was positively correlated with the number of cells injected. The reduction of lesion size could be assessed in vivo with MRI and MRS and was correlated with subsequent histological examination of the brain. This work demonstrates that highly purified hTERT-MSCs reduce cerebral infarction volume and improve functional outcome. |
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Authors:
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T Honma; O Honmou; S Iihoshi; K Harada; K Houkin; H Hamada; J D Kocsis |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2005-06-20 |
Journal Detail:
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Title: Experimental neurology Volume: 199 ISSN: 0014-4886 ISO Abbreviation: Exp. Neurol. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-06-26 Completed Date: 2006-08-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370712 Medline TA: Exp Neurol Country: United States |
Other Details:
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Languages: eng Pagination: 56-66 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Sapporo Medical University School of Medicine, South-1st, West-16th, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / metabolism Behavior, Animal Brain Ischemia / metabolism, pathology, physiopathology*, therapy* Cell Count / methods Cells, Cultured Disease Models, Animal Exercise Test / methods Green Fluorescent Proteins / metabolism Humans Immunohistochemistry / methods Infusions, Intravenous / methods Magnetic Resonance Imaging / methods Magnetic Resonance Spectroscopy / methods Male Maze Learning / physiology Mesenchymal Stem Cell Transplantation / methods* Mesenchymal Stem Cells / physiology* Phosphopyruvate Hydratase / metabolism Rats Rats, Sprague-Dawley Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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CA1009A10/CA/NCI NIH HHS; NS43432/NS/NINDS NIH HHS; R01 NS043432-05/NS/NINDS NIH HHS; RG2135/RG/CSR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 147336-22-9/Green Fluorescent Proteins; EC 4.2.1.11/Phosphopyruvate Hydratase |
| Comments/Corrections | |
Comment In:
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Exp Neurol. 2006 May;199(1):10-5
[PMID:
16730352
]
Exp Neurol. 2006 May;199(1):16-9 [PMID: 16696974 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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