| Intravenous or luminal amino acids are insufficient to maintain pancreatic growth and digestive enzyme expression in the absence of intact dietary protein. | |
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MedLine Citation:
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PMID: 20539007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported that rats receiving total parenteral nutrition (TPN) undergo significant pancreatic atrophy characterized by reduced total protein and digestive enzyme expression due to a lack of intestinal stimulation by nutrients (Baumler MD, Nelson DW, Ney DM, Groblewski GE. Am J Physiol Gastrointest Liver Physiol 292: G857-G866, 2007). Essentially identical results were recently reported in mice fed protein-free diets (Crozier SJ, D'Alecy LG, Ernst SA, Ginsburg LE, Williams JA. Gastroenterology 137: 1093-1101, 2009), provoking the question of whether reductions in pancreatic protein and digestive enzyme expression could be prevented by providing amino acids orally or by intravenous (IV) infusion while maintaining intestinal stimulation with fat and carbohydrate. Controlled studies were conducted in rats with IV catheters including orally fed/saline infusion or TPN-fed control rats compared with rats fed a protein-free diet, oral amino acid, or IV amino acid feeding, all with oral carbohydrate and fat. Interestingly, neither oral nor IV amino acids were sufficient to prevent the pancreatic atrophy seen for TPN controls or protein-free diets. Oral and IV amino acids partially attenuated the 75-90% reductions in pancreatic amylase and trypsinogen expression; however, values remained 50% lower than orally fed control rats. Lipase expression was more modestly reduced by a lack of dietary protein but did respond to IV amino acids. In comparison, chymotrypsinogen expression was induced nearly twofold in TPN animals but was not altered in other experimental groups compared with oral control animals. In contrast to pancreas, protein-free diets had no detectable effects on jejunal mucosal villus height, total mass, protein, DNA, or sucrase activity. These data underscore that, in the rat, intact dietary protein is essential in maintaining pancreatic growth and digestive enzyme adaptation but has surprisingly little effect on small intestinal mucosa. |
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Authors:
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Megan D Baumler; Matthew C Koopmann; Diana D H Thomas; Denise M Ney; Guy E Groblewski |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-06-10 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 299 ISSN: 1522-1547 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-29 Completed Date: 2010-08-30 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G338-47 Citation Subset: IM |
Affiliation:
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Dept. of Nutritional Sciences, Univ. of Wisconsin, Madison, 53706, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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drug effects Administration, Oral Amino Acids / administration & dosage* Amylases / metabolism Animals Atrophy Chymotrypsinogen / metabolism Diet, Protein-Restricted Dietary Proteins / metabolism* Growth Injections, Intravenous Intestinal Mucosa / drug effects Intestine, Small / drug effects Lipase / metabolism Male Pancreas / drug effects, pathology, physiopathology* Parenteral Nutrition, Total Protein Deficiency / physiopathology* Rats Rats, Sprague-Dawley Trypsinogen / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK-07088/DK/NIDDK NIH HHS; R01-DK-07665/DK/NIDDK NIH HHS; T32 DK-007665/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Dietary Proteins; 9002-08-8/Trypsinogen; 9035-75-0/Chymotrypsinogen; EC 3.1.1.3/Lipase; EC 3.2.1.-/Amylases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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