Document Detail

Intravenous glutamine enhances COX-2 activity giving cardioprotection.
MedLine Citation:
PMID:  18708191     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Preconditioning, a highly evolutionary conserved endogenous protective response, provides the most powerful form of anti-infarct protection known. We investigated whether acute intravenous glutamine, through an effect on cyclooxygenase (COX)-2 and heat shock protein (HSP) 72, might induce preconditioning. MATERIALS AND METHODS: Male New Zealand white rabbits (n = 28) received either 0.5 g/kg glutamine in 0.9% saline or saline only in divided doses for 3 d. The large marginal branch of the left coronary was occluded for 30 min; cardiac function was assessed during 3 h of reperfusion, and infarct size was measured. 6-Keto-PGF-1alpha, nitrate, and malonaldehyde serum levels were determined. Hearts were taken from a further group of pretreated rabbits (n = 10) to assess myocardial COX-2 and HSP72 levels. RESULTS: Glutamine pretreatment resulted in a 39% reduction in infarct size (30.7 +/- 2.0% versus 50.4 +/- 2.1% controls; P < 0.01). Myocardial COX-2 levels were significantly elevated with pretreatment (P < 0.05) and were mirrored by higher serum 6-keto-PGF-1alpha levels prior to ischemia (69 +/- 13 versus 18 +/- 21 pg/mL in controls, P = 0.027). There was no significant difference in myocardial HSP72 or serum nitrate levels following pretreatment, or malonaldehyde levels during reperfusion. CONCLUSIONS: Glutamine pretreatment confers anti-infarct protection through up-regulation of COX-2, a key mediator of delayed preconditioning protection. Previous confirmation of its clinical safety profile at these doses suggests an acceptable strategy for inducing preconditioning for perioperative protection.
Jonathan McGuinness; Tom G Neilan; Rob Cummins; Adel Sharkasi; David Bouchier-Hayes; J Mark Redmond
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-28
Journal Detail:
Title:  The Journal of surgical research     Volume:  152     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-11-03     Completed Date:  2009-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  140-7     Citation Subset:  IM    
The Royal College of Surgeons in Ireland, Department of Surgery, Beaumont Hospital, Dublin, Ireland.
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MeSH Terms
6-Ketoprostaglandin F1 alpha / blood
Cyclooxygenase 2 / metabolism*
Glutamine / administration & dosage*
HSP72 Heat-Shock Proteins / metabolism
Injections, Intravenous
Ischemic Preconditioning, Myocardial*
Malondialdehyde / blood
Myocardial Reperfusion Injury / pathology,  prevention & control*
Myocardium / metabolism,  pathology
Nitrates / blood
Reg. No./Substance:
0/HSP72 Heat-Shock Proteins; 0/Nitrates; 542-78-9/Malondialdehyde; 56-85-9/Glutamine; 58962-34-8/6-Ketoprostaglandin F1 alpha; EC 2

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