| Intravaginal Artificial Insemination in Bitches Using Frozen/Thawed Semen after Dilution in Powdered Coconut Water (ACP-106c). | |
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MedLine Citation:
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PMID: 23279521 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The aim of this study was to evaluate powdered coconut water extender (ACP-106c; ACP Serviços Tecnológicos Ltda, ACP Biotecnologia, Fortaleza, Ceará, Brazil) as a diluent for freezing dog semen and the fertility after vaginal insemination of semen frozen therein. Ten ejaculates were collected from five dogs, evaluated fresh, diluted in ACP-106c, 10% egg yolk and 6% glycerol, cooled and frozen. In the first phase of the study, straws with frozen semen were thawed and immediately subjected to the same analysis as the fresh semen and, in addition, to Computer-Assisted Semen Analysis (CASA). In phase 2, 10 bitches that had been subjected to natural breeding during a preceding oestrous cycle were vaginally inseminated with thawed semen that had been re-diluted in ACP-106c. After thawing, a mean of 77% sperm motility was obtained through subjective analysis and 77.3% through CASA. Following artificial insemination, a 60% pregnancy rate was observed, resulting in a 50% parturition rate and a mean litter size of 3.4 (SEM 0.6), with 47.1% males and 52.9% females. ACP-106c can be successfully used for freezing canine semen, and vaginal deposition of such semen yields similar pregnancy rates to those reported in other studies. |
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Authors:
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Dc Uchoa; Tfp Silva; Ac Mota Filho; Ldm Silva |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction in domestic animals = Zuchthygiene Volume: 47 Suppl 6 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2013-01-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 289-92 Citation Subset: IM |
Copyright Information:
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© 2012 Blackwell Verlag GmbH. |
Affiliation:
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Grande Canafístula Kennel/Inseminar Reprodução Animal, Fortaleza, Brazil. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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