Document Detail


Intratracheal administration of endotoxin and cytokines. VII. The soluble interleukin-1 receptor and the soluble tumor necrosis factor receptor II (p80) inhibit acute inflammation.
MedLine Citation:
PMID:  8020186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intratracheal administration of endotoxin (LPS) causes acute neutrophilic inflammation via induction of pulmonary tumor necrosis factor alpha (TNF) and interleukin-1 (IL-1) expression. In the present study, the anti-inflammatory activity of soluble IL-1 receptor (sIL-1r) and soluble TNF receptor p80 (sTNFr-p80) in LPS-induced acute pulmonary inflammation was investigated. The sIL-1r coinjected intratracheally with LPS in rats significantly inhibits neutrophilic exudation into bronchoalveolar lavage (BAL) fluid by 47% after 6 hr compared to injection of LPS alone. TNF and IL-6 in the same BAL fluids were both lowered by approximately 50% after intratracheal coinjection of sIL-1r and LPS as compared to LPS alone. In the same model, the sTNFr-p80 inhibited acute inflammation. Paradoxically, TNF levels in BAL fluids were generally elevated after the intratracheal coinjection of LPS and monomeric sTNFr-p80 compared to injection of LPS injection alone. The combined anti-inflammatory effect of sIL-1r and sTNFr-p80 at the maximally effective individual doses is not significantly greater than the effect of either soluble receptor alone.
Authors:
T R Ulich; E S Yi; S Yin; C Smith; D Remick
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical immunology and immunopathology     Volume:  72     ISSN:  0090-1229     ISO Abbreviation:  Clin. Immunol. Immunopathol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-07-29     Completed Date:  1994-07-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0356637     Medline TA:  Clin Immunol Immunopathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  137-40     Citation Subset:  IM    
Affiliation:
Department of Pathology, San Diego School of Medicine, University of California.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bronchoalveolar Lavage Fluid / immunology
Endotoxins / administration & dosage,  antagonists & inhibitors*
Inflammation / immunology,  prevention & control*
Interleukin-6 / biosynthesis
Intubation, Intratracheal
Lipopolysaccharides / antagonists & inhibitors
Male
Rats
Rats, Inbred Lew
Receptors, Interleukin-1*
Receptors, Tumor Necrosis Factor*
Recombinant Proteins / administration & dosage,  pharmacology
Solubility
Tumor Necrosis Factor-alpha / biosynthesis
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Receptors, Interleukin-1; 0/Receptors, Tumor Necrosis Factor; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha

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