Document Detail


Intrathecal injection of TRPV1 shRNA leads to increases in blood pressure in rats.
MedLine Citation:
PMID:  21518266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The transient receptor potential vanilloid type 1 (TRPV1) channels have been implicated to play a role in blood pressure regulation. However, contribution of tissue specific TRPV1 to blood pressure regulation is largely unknown. Here, we test the hypothesis that TRPV1 expressed in dorsal root ganglia (DRG) of lower thoracic and upper lumbar segments (T8-L3) of the spinal cord and their central and peripheral terminals constitutes a counter regulatory mechanism preventing the increases in blood pressure.
METHODS: The expression of TRPV1 was knocked down by intrathecal injection of TRPV1 short-hairpin RNA (shRNA) in rats. Systolic blood pressure and mean arterial pressure (MAP) were recorded. The level of TRPV1 and tyrosine hydroxylase (TH) was measured by Western blot.
RESULTS: Intrathecal injection of TRPV1 shRNA (6 μg kg(-1) day(-1) ) for 3 days increased systolic blood pressure and MAP when compared to rats that received control shRNA (control shRNA: 112 ± 2 vs. TRPV1 shRNA: 123 ± 2 mmHg). TRPV1 expression was suppressed in T8-L3 segments of dorsal horn and DRG as well as mesenteric arteries of rats given TRPV1 shRNA. Contents of TH, a marker of sympathetic nerves, were increased in mesenteric arteries of rats treated with TRPV1 shRNA. Pretreatment with the α1-adrenoceptor blocker, prazosin (1 mg kg(-1) day(-1) , p.o.), abolished the TRPV1 shRNA-induced pressor effects.
CONCLUSION: Our data show that selective knockdown of TRPV1 expressed in DRG of T8-L3 segments of the spinal cord and their central and peripheral terminals increases blood pressure, suggesting that neuronal TRPV1 in these segments possesses a tonic anti-hypertensive effect possibly via suppression of the sympathetic nerve activity.
Authors:
S-Q Yu; D H Wang
Related Documents :
21138476 - Melatonin treatment improves blood pressure, lipid profile, and parameters of oxidative...
21456766 - Preparation and accurate measurement of pure ozone.
21406796 - Structural compression and vibrational properties of bi(12)sio(20) sillenite from exper...
21165806 - Effects of isometric handgrip protocol on blood pressure and neurocardiac modulation.
14511846 - Comparative effects of lercanidipine, lacidipine, and nifedipine gastrointestinal thera...
8157946 - Structural adaptation of the heart in borderline hypertensives in response to blood pre...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-04-22
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  203     ISSN:  1748-1716     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-16     Completed Date:  2011-12-07     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  139-47     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Acta Physiologica © 2011 Scandinavian Physiological Society.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / drug effects*
Blotting, Western
Ganglia, Spinal / drug effects*,  metabolism
Gene Knockdown Techniques / methods
Immunohistochemistry
Injections, Spinal
Male
RNA, Small Interfering / administration & dosage*
Rats
Rats, Wistar
Sympathetic Nervous System / drug effects,  metabolism
TRPV Cation Channels / antagonists & inhibitors*,  biosynthesis,  genetics
Grant Support
ID/Acronym/Agency:
DK67620/DK/NIDDK NIH HHS; HL-57853/HL/NHLBI NIH HHS; HL-73287/HL/NHLBI NIH HHS; R01 DK067620/DK/NIDDK NIH HHS; R01 DK067620-05/DK/NIDDK NIH HHS; R01 HL057853/HL/NHLBI NIH HHS; R01 HL057853-07/HL/NHLBI NIH HHS; R01 HL073287/HL/NHLBI NIH HHS; R01 HL073287-07/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Small Interfering; 0/TRPV Cation Channels; 0/Trpv1 protein, rat
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of ageing and endurance exercise training on alpha-actinin isoforms in rat plantaris muscle.
Next Document:  Wnt5a: its signalling, functions and implication in diseases.