Document Detail


Intrastriatal B-cell administration limits infarct size after stroke in B-cell deficient mice.
MedLine Citation:
PMID:  22618587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent evidence emphasizes B-cells as a major regulatory cell type that plays an important role in limiting the pathogenic effects of ischemic stroke. The aim of the current study was to extend this initial observation to specifically examine the infiltration of regulatory B-cells and to determine if the effect of B-cells to limit the inflammatory response to cerebral ischemia is mediated by their action centrally or peripherally. Our data demonstrate the increased presence of a regulatory B-cell subset in the affected hemisphere of wild-type mice after middle cerebral artery occlusion (MCAO). We further explored the use of a novel method of stereotaxic cell delivery to bypass the blood brain barrier (BBB) and introduce CD19(+) B-cells directly into the striatum as compared to peripheral administration of B-cells. Infarct volumes after 60 minutes of MCAO and 48 hours of reperfusion were determined in B-cell deficient μMT( -/- ) mice with and without replacement of either B-cells or medium. Infarct size was significantly decreased in cerebral cortex after intrastriatal transfer of 100,000 B-cells to μMT(-/-) mice vs. controls, with a comparable effect on infarct size as obtained by 50 million B-cells transferred intraperitoneally. These findings support the hypothesis that B-cells play a protective role against ischemic brain injury, and suggest that B-cells may serve as a novel therapeutic agent for modulating the immune response in central nervous system inflammation after stroke.
Authors:
Yingxin Chen; Sheetal Bodhankar; Stephanie J Murphy; Arthur A Vandenbark; Nabil J Alkayed; Halina Offner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-05-18
Journal Detail:
Title:  Metabolic brain disease     Volume:  27     ISSN:  1573-7365     ISO Abbreviation:  Metab Brain Dis     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-05-03     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  8610370     Medline TA:  Metab Brain Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  487-93     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD19
B-Lymphocytes, Regulatory / physiology*
Cell Separation
Cell Transplantation*
Cerebellar Cortex / pathology
Cerebral Infarction / pathology*,  therapy*
Fluorescent Antibody Technique
Immunohistochemistry
Laser-Doppler Flowmetry
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Middle Cerebral Artery / physiology
Neostriatum / physiology
Stereotaxic Techniques
Stroke / pathology*,  therapy*
Grant Support
ID/Acronym/Agency:
NS075887/NS/NINDS NIH HHS; R01 NS044313/NS/NINDS NIH HHS; R01 NS070837/NS/NINDS NIH HHS; R01 NS075887/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD19
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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