Document Detail

Intraspecific variability of Lactarius deliciosus isolates: colonization ability and survival after cold storage.
MedLine Citation:
PMID:  21120543     Owner:  NLM     Status:  In-Data-Review    
Intraspecific variability in root colonization, extraradical growth pattern, and survival after cold storage of Lactarius deliciosus isolates was determined in pure culture conditions using Pinus pinaster as a host plant. The ectomycorrhizal ability of L. deliciosus at 30, 45, and 60 days from inoculation was highly variable among isolates and was negatively correlated to the age of the culture (time elapsed from isolation). The formation of rhizomorphs was related to colonization ability, but no relationship was found between colonization and formation of extraradical mycelium. The final colonization achieved at 60 days from inoculation was not related to the tree species under which the sporocarps were collected. However, isolates from sporocarps collected under P. pinaster colonized more rapidly the seedlings than those collected under other pine species. The climatic range of the sporocarps from which the isolates were obtained (maritime vs. continental) was not related to the formation of mycorrhizas at 60 days from inoculation. However, isolates from sporocarps collected from a maritime climate area colonized more rapidly the P. pinaster seedlings than those collected from a continental zone. Tolerance to cold water storage of L. deliciosus was also isolate dependent. Growth revival in agar was obtained from most of the isolates after 28 months of cold storage at 4°C, but only 10 out of 29 isolates showed unaffected growth. The ITS rDNA alignment of all the L. deliciosus isolates showed a low variability with identities over 99%. Most of the variation was detected in the ITS1 region and consisted in single nucleotide changes and/or punctual indel mutations. The number of base differences per sequence from averaging over all sequence pairs was 1.329, which is in the low range when compared with other ectomycorrhizal species. No ITS pattern due to geographical origin of the isolates could be discerned.
Javier Parladé; Sara Hortal; Herminia de la Varga; Joan Pera
Related Documents :
24334863 - In vitro susceptibility of malassezia pachydermatis isolates from canine skin with atop...
18616273 - Lc-ms analysis of solanidane glycoalkaloid diversity among tubers of four wild potato s...
21045363 - Disseminated cryptococcosis and fluconazole resistant oral candidiasis in a patient wit...
Publication Detail:
Type:  Journal Article     Date:  2010-12-01
Journal Detail:
Title:  Mycorrhiza     Volume:  21     ISSN:  1432-1890     ISO Abbreviation:  Mycorrhiza     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955036     Medline TA:  Mycorrhiza     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  393-401     Citation Subset:  IM    
IRTA, Patologia Vegetal, Centre de Cabrils, Ctra. Cabrils Km 2, 08348, Cabrils, Barcelona, Spain,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Comparison of Supreme(®) and Soft Seal (®) laryngeal masks for airway management during cardiopulmon...
Next Document:  Transportability of the updated diabetes prediction model from Atherosclerosis Risk in Communities S...