Document Detail

Intraspecific phylogeography of the gopher tortoise, Gopherus polyphemus: RFLP analysis of amplified mtDNA segments.
MedLine Citation:
PMID:  8564009     Owner:  NLM     Status:  MEDLINE    
The slow rate of mtDNA evolution in turtles poses a limitation on the levels of intraspecific variation detectable by conventional restriction fragment surveys. We examined mtDNA variation in the gopher tortoise (Gopherus polyphemus) using an alternative restriction assay, one in which PCR-amplified segments of the mitochondrial genome were digested with tetranucleotide-site endonucleases. Restriction fragment polymorphisms representing four amplified regions were analysed to evaluate population genetic structure among 112 tortoises throughout the species' range. Thirty-six haplotypes were identified, and three major geographical assemblages (Eastern, Western, and Mid-Florida) were resolved by UPGMA and parsimony analyses. Eastern and Western assemblages abut near the Apalachicola drainage, whereas the Mid-Florida assemblage appears restricted to the Brooksville Ridge. The Eastern/Western assemblage boundary is remarkably congruent with phylogeographic profiles for eight additional species from the south-eastern U.S., representing both freshwater and terrestrial realms.
M F Osentoski; T Lamb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular ecology     Volume:  4     ISSN:  0962-1083     ISO Abbreviation:  Mol. Ecol.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-03-05     Completed Date:  1996-03-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9214478     Medline TA:  Mol Ecol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  709-18     Citation Subset:  IM    
Department of Biology, East Carolina University, Greenville, NC 27858, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U22811;  U22812;  U22813;  U22814
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MeSH Terms
Base Sequence
DNA Primers / genetics
DNA, Mitochondrial / genetics*
Genetic Variation
Genetics, Population
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Southeastern United States
Turtles / genetics*
Reg. No./Substance:
0/DNA Primers; 0/DNA, Mitochondrial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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