Document Detail

Intrascleral drug delivery to the eye using hollow microneedles.
MedLine Citation:
PMID:  18979189     Owner:  NLM     Status:  MEDLINE    
PURPOSE: This study tested the hypothesis that hollow microneedles can infuse solutions containing soluble molecules, nanoparticles, and microparticles into sclera in a minimally invasive manner.
METHODS: Individual hollow microneedles were inserted into, but not across, human cadaver sclera and aqueous solutions containing sulforhodamine or fluorescently tagged nanoparticles or microparticles were infused into sclera at constant pressure. The infused volume of fluid was measured and imaged histologically as a function of scleral thickness, infusion pressure, needle retraction depth and the presence of spreading enzymes (hyaluronidase and collagenase).
RESULTS: Individual hollow microneedles were able to insert into sclera. Fluid infusion was extremely slow after microneedle insertion into the sclera without retraction, but partial retraction of the microneedle over a distance of 200-300 microm enabled infusion of 10-35 microl of fluid into the tissue. Scleral thickness and infusion pressure had insignificant effects on fluid delivery. Nanoparticle suspensions were also delivered into sclera, but microparticles were delivered only in the presence of hyaluronidase and collagenase spreading enzymes, which suggested the role of scleral glycosaminoglycans and collagen fibers as rate-limiting barriers.
CONCLUSION: This study shows that hollow microneedles can infuse solutions into the sclera for minimally invasive delivery of soluble molecules, nanoparticles and microparticles.
Jason Jiang; Jason S Moore; Henry F Edelhauser; Mark R Prausnitz
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural     Date:  2008-11-01
Journal Detail:
Title:  Pharmaceutical research     Volume:  26     ISSN:  0724-8741     ISO Abbreviation:  Pharm. Res.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-19     Completed Date:  2009-03-23     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  395-403     Citation Subset:  IM    
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MeSH Terms
Collagenases / metabolism
Equipment Design
Fibrillar Collagens / metabolism
Glycosaminoglycans / metabolism
Hyaluronoglucosaminidase / metabolism
Microinjections / instrumentation*
Particle Size
Rhodamines / administration & dosage*,  chemistry,  metabolism
Sclera / metabolism*
Grant Support
R01 EB000260/EB/NIBIB NIH HHS; R01 EB000260-03/EB/NIBIB NIH HHS; R01 EB000260-04/EB/NIBIB NIH HHS; R01 GM060004/GM/NIGMS NIH HHS; R01 GM060004-01A1/GM/NIGMS NIH HHS; R01 GM060004-02/GM/NIGMS NIH HHS; R01 GM060004-03/GM/NIGMS NIH HHS; R24 EY017045-01/EY/NEI NIH HHS; R24 EY017045-02/EY/NEI NIH HHS; R24 EY017045-03/EY/NEI NIH HHS; R24-EY-017045/EY/NEI NIH HHS
Reg. No./Substance:
0/Fibrillar Collagens; 0/Glycosaminoglycans; 0/Rhodamines; EC; EC 3.4.24.-/Collagenases; EC 3.4.24.-/collagenase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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