| Intrarenal urothelium proliferation: an unexpected early event following ischemic injury. | |
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MedLine Citation:
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PMID: 20591940 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Identification of renal cell progenitors and recognition of the events contributing to cell regeneration following ischemia-reperfusion injury (IRI) are a major challenge. In a mouse model of unilateral renal IRI, we demonstrated that the first cells to proliferate within injured kidneys were urothelial cells expressing the progenitor cell marker cytokeratin 14. A systematic cutting of the injured kidney revealed that these urothelial cells were located in the deep cortex at the corticomedullary junction in the vicinity of lobar vessels. Contrary to multilayered bladder urothelium, these intrarenal urothelial cells located in the upper part of the medulla constitute a monolayered barrier and express among uroplakins only uroplakin III. However, like bladder progenitors, intrarenal urothelial cells proliferated through a FGF receptor-2 (FGFR2)-mediated process. They strongly expressed FGFR2 and proliferated in vivo after recombinant FGF7 administration to control mice. In addition, IRI led to FGFR phosphorylation together with the selective upregulation of FGF7 and FGF2. Conversely, by day 2 following IRI, renal urothelial cell proliferation was significantly inhibited by FGFR2 antisense oligonucleotide administration into an intrarenal urinary space. Of notice, no significant migration of these early dividing urothelial cells was detected in the cortex within 7 days following IRI. Thus our data show that following IRI, proliferation of urothelial cells is mediated by the FGFR2 pathway and precedes tubular cell proliferation, indicating a particular sensitivity of this structure to changes caused by the ischemic process. |
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Authors:
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C Vinsonneau; A Girshovich; M Ben M'rad; J Perez; L Mesnard; S Vandermersch; S Placier; E Letavernier; L Baud; J-P Haymann |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-30 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: 299 ISSN: 1522-1466 ISO Abbreviation: Am. J. Physiol. Renal Physiol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-03 Completed Date: 2010-09-24 Revised Date: 2011-04-28 |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: United States |
Other Details:
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Languages: eng Pagination: F479-86 Citation Subset: IM |
Affiliation:
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INSERM UMRS, Université Pierre et Marie Curie Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Proliferation* Disease Models, Animal Female Fibroblast Growth Factor 7 / physiology Kidney Cortex / pathology* Mice Mice, Inbred C57BL Receptor, Fibroblast Growth Factor, Type 2 / physiology Reperfusion Injury / pathology*, physiopathology Signal Transduction / physiology Urothelium / pathology |
| Chemical | |
Reg. No./Substance:
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0/Fgf7 protein, mouse; 126469-10-1/Fibroblast Growth Factor 7; EC 2.7.1.112/Fgfr2 protein, mouse; EC 2.7.10.1/Receptor, Fibroblast Growth Factor, Type 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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