Document Detail

Intrarenal ghrelin infusion stimulates distal nephron-dependent sodium reabsorption in normal rats.
MedLine Citation:
PMID:  21220707     Owner:  NLM     Status:  MEDLINE    
Ghrelin is a 28-amino acid peptide hormone that exerts powerful orexigenic effects. Ghrelin receptor expression has been reported in the kidney, but the role of ghrelin in the kidney is unknown. The present studies confirmed ghrelin receptor mRNA expression in the kidneys of normal Sprague Dawley rats (n=6) using reverse transcription polymerase chain reaction (PCR) and sequencing of the 588-bp PCR product. To test intrarenal ghrelin action, uninephrectomized rats received 3 cumulative 1-hour renal interstitial (RI) infusions of 5% dextrose in water (vehicle, n=21), ghrelin (n=10), ghrelin plus specific ghrelin receptor antagonist [D-Lys-3]-GHRP-6 (n=24), or [D-Lys-3]-GHRP-6 alone (n=32). Mean arterial pressure (MAP), urine sodium excretion rate (U(Na)V), glomerular filtration rate (GFR), fractional excretion of sodium (FE(Na)), and fractional excretion of lithium (FE(Li)) were calculated for each period. RI ghrelin infusion significantly decreased U(Na)V to 86 ± 4.9% (P<0.05), 74 ± 6.5% (P<0.01), and 62 ± 10% (P<0.01) of baseline during periods 1 to 3, respectively. Ghrelin also significantly decreased FE(Na) to 68 ± 11% (P<0.05), 57 ± 8.6% (P<0.001), and 59 ± 12% (P<0.01) of baseline, without changing GFR or FE(Li). Identical ghrelin infusions in the presence of [D-Lys-3]-GHRP-6 failed to permit reductions in U(Na)V or FE(Na). Following [D-Lys-3]-GHRP-6 infusion alone, U(Na)V increased from 0.06 ± 0.01 to 0.18 ± 0.03 μmol/min (P<0.0001). Concomitant increases in FE(Na) were also observed (0.23 ± 0.03% to 0.51 ± 0.06% [P<0.001]), without changes in MAP, GFR, or FE(Li). Together, these data introduce a novel intrarenal ghrelin-ghrelin receptor system, which, on activation, significantly increases Na(+) reabsorption at the level of the distal nephron.
Brandon A Kemp; Nancy L Howell; Jasmine T Gray; Susanna R Keller; Ralf M Nass; Shetal H Padia
Related Documents :
21376127 - Functional biomarkers of musculoskeletal syndrome (mss) for early in vivo screening of ...
10398377 - Temporal patterns of dna adduct formation and glutathione s-transferase activity in the...
21354297 - Antidepressant effect of valeriana wallichii patchouli alcohol chemotype in mice: behav...
21225017 - Effect of the at₁ receptor antagonist losartan on diurnal variation in pain threshold...
20021037 - Aluminium-induced imbalance in oxidant and antioxidant determinants in brain regions of...
1844317 - Stress-induced change in cerebral atp+ubiquitin-dependent proteinase activity vary with...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-10
Journal Detail:
Title:  Hypertension     Volume:  57     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-17     Completed Date:  2011-04-14     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  United States    
Other Details:
Languages:  eng     Pagination:  633-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Analysis of Variance
Blood Pressure / drug effects*,  physiology
Ghrelin / metabolism,  pharmacology*
Glomerular Filtration Rate / drug effects,  physiology
Kidney / drug effects*,  metabolism
Natriuresis / drug effects,  physiology
Nephrons / drug effects*,  metabolism
Oligopeptides / pharmacology
Rats, Sprague-Dawley
Receptors, Ghrelin / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sodium / metabolism*
Grant Support
5K08HL093353/HL/NHLBI NIH HHS; K08 HL093353/HL/NHLBI NIH HHS; K08 HL093353-02/HL/NHLBI NIH HHS; K08 HL093353-03/HL/NHLBI NIH HHS
Reg. No./Substance:
0/GHRP-6, Lys(3)-; 0/Ghrelin; 0/Oligopeptides; 0/Receptors, Ghrelin; 9NEZ333N27/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A murine model of isolated cardiac steatosis leads to cardiomyopathy.
Next Document:  Nuclear factor kappa B and matrix metalloproteinase induced receptor cleavage in the spontaneously h...