| Intrarenal ghrelin infusion stimulates distal nephron-dependent sodium reabsorption in normal rats. | |
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MedLine Citation:
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PMID: 21220707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ghrelin is a 28-amino acid peptide hormone that exerts powerful orexigenic effects. Ghrelin receptor expression has been reported in the kidney, but the role of ghrelin in the kidney is unknown. The present studies confirmed ghrelin receptor mRNA expression in the kidneys of normal Sprague Dawley rats (n=6) using reverse transcription polymerase chain reaction (PCR) and sequencing of the 588-bp PCR product. To test intrarenal ghrelin action, uninephrectomized rats received 3 cumulative 1-hour renal interstitial (RI) infusions of 5% dextrose in water (vehicle, n=21), ghrelin (n=10), ghrelin plus specific ghrelin receptor antagonist [D-Lys-3]-GHRP-6 (n=24), or [D-Lys-3]-GHRP-6 alone (n=32). Mean arterial pressure (MAP), urine sodium excretion rate (U(Na)V), glomerular filtration rate (GFR), fractional excretion of sodium (FE(Na)), and fractional excretion of lithium (FE(Li)) were calculated for each period. RI ghrelin infusion significantly decreased U(Na)V to 86 ± 4.9% (P<0.05), 74 ± 6.5% (P<0.01), and 62 ± 10% (P<0.01) of baseline during periods 1 to 3, respectively. Ghrelin also significantly decreased FE(Na) to 68 ± 11% (P<0.05), 57 ± 8.6% (P<0.001), and 59 ± 12% (P<0.01) of baseline, without changing GFR or FE(Li). Identical ghrelin infusions in the presence of [D-Lys-3]-GHRP-6 failed to permit reductions in U(Na)V or FE(Na). Following [D-Lys-3]-GHRP-6 infusion alone, U(Na)V increased from 0.06 ± 0.01 to 0.18 ± 0.03 μmol/min (P<0.0001). Concomitant increases in FE(Na) were also observed (0.23 ± 0.03% to 0.51 ± 0.06% [P<0.001]), without changes in MAP, GFR, or FE(Li). Together, these data introduce a novel intrarenal ghrelin-ghrelin receptor system, which, on activation, significantly increases Na(+) reabsorption at the level of the distal nephron. |
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Authors:
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Brandon A Kemp; Nancy L Howell; Jasmine T Gray; Susanna R Keller; Ralf M Nass; Shetal H Padia |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-01-10 |
Journal Detail:
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Title: Hypertension Volume: 57 ISSN: 1524-4563 ISO Abbreviation: Hypertension Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-17 Completed Date: 2011-04-14 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 7906255 Medline TA: Hypertension Country: United States |
Other Details:
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Languages: eng Pagination: 633-9 Citation Subset: IM |
Affiliation:
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Division of Endocrinology and Metabolism, Department of Medicine, University of Virginia Health System, Charlottesville, VA 22908-1414, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Blood Pressure / drug effects*, physiology Female Ghrelin / metabolism, pharmacology* Glomerular Filtration Rate / drug effects, physiology Kidney / drug effects*, metabolism Natriuresis / drug effects, physiology Nephrons / drug effects*, metabolism Oligopeptides / pharmacology Rats Rats, Sprague-Dawley Receptors, Ghrelin / metabolism Reverse Transcriptase Polymerase Chain Reaction Sodium / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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5K08HL093353/HL/NHLBI NIH HHS; K08 HL093353-02/HL/NHLBI NIH HHS; K08 HL093353-03/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/GHRP-6, Lys(3)-; 0/Ghrelin; 0/Oligopeptides; 0/Receptors, Ghrelin; 7440-23-5/Sodium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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