| Intraperitoneal recombinant interleukin-2 activates leukocytes in rat endometriosis. | |
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MedLine Citation:
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PMID: 17210185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this double-blinded study was to determine changes in leukocyte populations in blood, peritoneal lavage fluid, eutopic and ectopic endometrium after treatment with recombinant rat interleukin-2 (IL-2) using an in vivo experimental model of rat endometriosis. The in vivo model involved transplanting four square fragments of autologous endometrium onto the inner surface of the abdominal wall in 20 Wistar rats. The control group was constituted by 20 sham-operated rats. Both groups were randomly treated (1-month interval treatment) with 2 intraperitoneal doses of glucose solution (5%) that did or did not contain recombinant IL-2, and animals were sacrificed 4 weeks after the last dose of treatment. Blood and peritoneal lavage were obtained during the initial and final laparotomy, whereas eutopic and ectopic endometrium were collected at the end of the experiment. Endometriotic implants were measured in each laparotomy to determine any change in size. Leukocyte populations were analyzed by flow cytometry and immunofluorescence microscopy. Cytometric results were similar in blood and peritoneal lavage. CD25+ and natural killer (NK) cell levels in peripheral blood were lower in rats with endometriosis treated with IL-2, whereas NK cells increased in lavage compared to placebo group. The percentage of macrophages and dendritic cells in blood were higher in all rats treated with IL-2, as well as peritoneal dendritic cells. Implant size of these rats decreased significantly, showing a greater number of activated lymphocytes, macrophages, NK and dendritic cells inside them. In conclusion, recombinant IL-2 induced recruitment of activated leukocytes into endometriotic-like foci, and this was related to a reduction of the implant size, suggesting potential effectiveness of IL-2 as an immunomodulatory agent in this pathology. |
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Authors:
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Irene Velasco; Francisco Quereda; Rosa Bermejo; Adolfo Campos; Pedro Acién |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-08 |
Journal Detail:
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Title: Journal of reproductive immunology Volume: 74 ISSN: 0165-0378 ISO Abbreviation: J. Reprod. Immunol. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-05-09 Completed Date: 2007-07-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8001906 Medline TA: J Reprod Immunol Country: Ireland |
Other Details:
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Languages: eng Pagination: 124-32 Citation Subset: IM |
Affiliation:
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Division of Gynecology, School of Medicine, Miguel Hernández University, Alicante, Spain. ivelasco@umh.es |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Double-Blind Method Endometriosis / drug therapy*, immunology Female Flow Cytometry Immunohistochemistry Injections, Intraperitoneal Interleukin-2 / administration & dosage, immunology, therapeutic use* Killer Cells, Natural / drug effects, immunology* Leukocytes / drug effects, immunology* Random Allocation Rats Rats, Wistar Recombinant Proteins / administration & dosage, therapeutic use |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-2; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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