Document Detail

Intraperitoneal administration of hyperbarically oxygenated perfluorochemical enhances preservation of intestinal mucosa against ischemia/reperfusion injury.
MedLine Citation:
PMID:  17117139     Owner:  NLM     Status:  MEDLINE    
Perfluorochemicals (PFCs) have a high solubility for oxygen. We have previously demonstrated the effect of peritoneal lavage with oxygenated PFC (O2-PFC) on ameliorating ischemia/reperfusion (I/R)-induced intestinal ischemic damage in an animal model. In this study, we applied hyperbarically O2-PFC (HBO-PFC) to investigate whether a larger amount of oxygen carried by PFC could enhance the protective effect of O2-PFC during intestinal malperfusion. Rats were subjected to ischemia by clamping the superior mesenteric artery (SMA) for 90 min. The SMA was then declamped. Rats were divided into four groups. In group A, only anesthesia and abdominal incision were performed. In group B, SMA was clamped without O2-PFC. In group C, during the SMA clamp, 1 atm O2-PFC was injected into the abdominal cavity. In group D, 5 atm O2-PFC (HBO-PFC) was prepared using a custom-made hyperbaric oxygen tank and administered to the abdominal cavity during the SMA clamp. Ileal tissue adenosine triphosphate (ATP) levels 90 min after SMA declamping were determined using luciferase assay. To assess intestinal mucosal barrier function at 90 min after release of the SMA clip, everted gut sacs were prepared to measure the mucosal-to-serosal passage of fluorescein-conjugated dextran (FD4, molecular weight = 4 kDa). Thirty minutes after i.p. administration, partial pressure of oxygen in HBO-PFC remained around 1000 mmHg, whereas partial pressure of oxygen in 1 atm O2-PFC decreased to around 400 mmHg. The intestinal tissue ATP was significantly preserved in group D. Moreover, the mucosal hyperpermeability of the gut sac after I/R was significantly ameliorated in group D. Hyperbarically oxygenated perfluorochemical might supply a larger amount of oxygen to ischemic tissue during SMA clamp, which protected the small intestine from I/R injury, possibly caused by the maintenance of tissue ATP levels during ischemia.
Takaaki Saito; Naoki Unno; Naoto Yamamoto; Kazunori Inuzuka; Daisuke Sagara; Minoru Suzuki; Hiroyuki Konno
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Publication Detail:
Type:  Historical Article; Journal Article    
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  26     ISSN:  1073-2322     ISO Abbreviation:  Shock     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-22     Completed Date:  2007-01-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  United States    
Other Details:
Languages:  eng     Pagination:  620-4     Citation Subset:  IM    
Second Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan.
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MeSH Terms
Adenosine Triphosphate / metabolism
Fluorocarbons / administration & dosage*,  pharmacology*
Furans / administration & dosage*,  pharmacology*
History, 15th Century
Hyperbaric Oxygenation*
Interleukin-6 / metabolism
Intestinal Mucosa / drug effects*,  pathology*
Intestines / metabolism
Multiple Organ Failure / drug therapy
Oxygen / metabolism
Rats, Sprague-Dawley
Reperfusion Injury / drug therapy*
Time Factors
Reg. No./Substance:
0/Fluorocarbons; 0/Furans; 0/Interleukin-6; 26446-59-3/perfluorobutyl tetrahydrofuran; 56-65-5/Adenosine Triphosphate; 7782-44-7/Oxygen

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