Document Detail

Intrapatient sequence variation of the gag gene of human immunodeficiency virus type 1 plasma virions.
MedLine Citation:
PMID:  8971017     Owner:  NLM     Status:  MEDLINE    
Because certain regions of the gag gene, such as p24, are highly conserved among human immunodeficiency virus (HIV) isolates, many therapeutic strategies have been directed at gag gene targets. Although intrapatient variation of segments of gag have been determined, little is known about the variability of the full-length gag gene for HIV isolated from a single individual. To evaluate intrapatient full-length gag variability, we derived the nucleotide sequences of at least 10 cDNA gag clones of virion RNA isolated from plasma for each of four asymptomatic HIV type 1-infected patients with relatively high CD4+ T-cell counts (300 to 450 cells per mm3). Mean values of intrapatient gag nucleotide variation obtained by pairwise comparisons ranged from 0.55 to 2.86%. For three subjects, this value was equivalent to that reported for intrapatient full-length env variation. The greatest range of intrapatient mean nucleotide variation for individual protein-coding regions was observed for p7. We did not detect any G-to-A hypermutation, as A-to-G and G-to-A transitions occurred at similar frequencies, accounting for 29 and 25%, respectively, of the changes. Mean variation values and phylogenetic analysis suggested that the extent of nucleotide variation correlated with the length of viral infection. Furthermore, no distinct subpopulations of quasispecies were detectable within an individual. The predicted amino acid sequences indicated that there were no regions within a gag protein that were comprised of clustered changes.
F K Yoshimura; K Diem; G H Learn; S Riddell; L Corey
Related Documents :
1870207 - Activation of the c-ski oncogene by overexpression.
9837787 - Phylogeographic patterns of feline immunodeficiency virus genetic diversity in the dome...
2435057 - Sequence relationships of type d retroviruses which cause simian acquired immunodeficie...
1322587 - Analysis of a human immunodeficiency virus type 1 isolate carrying a truncated transmem...
17697377 - Phylogenomic analyses of kcna gene clusters in vertebrates: why do gene clusters stay i...
20679667 - Co-segregation of norrie disease and idiopathic pulmonary hypertension in a family with...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  70     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-23     Completed Date:  1997-01-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  8879-87     Citation Subset:  IM; X    
Department of Biological Structure, University of Washington, Seattle 98195, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U29242;  U29246;  U29247;  U29248;  U29249;  U29250;  U29251;  U29252;  U29253;  U29254;  U29255;  U29256;  U29257;  U29258;  U29259;  U29260;  U29261;  U29262;  U29263;  U29264;  U29265;  U29403;  U29404;  U29405;  U29406;  U29407;  U29408;  U29409;  U29410;  U29411
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Base Sequence
DNA, Viral
Genes, gag*
Genetic Variation*
HIV Infections / blood,  virology*
HIV-1 / classification,  genetics*,  isolation & purification
Molecular Sequence Data
Sequence Homology, Amino Acid
Grant Support
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A hypervariable region in VP1 of chicken infectious anemia virus mediates rate of spread and cell tr...
Next Document:  Pathogenesis of murine enterovirus myocarditis: virus dissemination and immune cell targets.