| Intrapatient sequence variation of the gag gene of human immunodeficiency virus type 1 plasma virions. | |
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MedLine Citation:
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PMID: 8971017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Because certain regions of the gag gene, such as p24, are highly conserved among human immunodeficiency virus (HIV) isolates, many therapeutic strategies have been directed at gag gene targets. Although intrapatient variation of segments of gag have been determined, little is known about the variability of the full-length gag gene for HIV isolated from a single individual. To evaluate intrapatient full-length gag variability, we derived the nucleotide sequences of at least 10 cDNA gag clones of virion RNA isolated from plasma for each of four asymptomatic HIV type 1-infected patients with relatively high CD4+ T-cell counts (300 to 450 cells per mm3). Mean values of intrapatient gag nucleotide variation obtained by pairwise comparisons ranged from 0.55 to 2.86%. For three subjects, this value was equivalent to that reported for intrapatient full-length env variation. The greatest range of intrapatient mean nucleotide variation for individual protein-coding regions was observed for p7. We did not detect any G-to-A hypermutation, as A-to-G and G-to-A transitions occurred at similar frequencies, accounting for 29 and 25%, respectively, of the changes. Mean variation values and phylogenetic analysis suggested that the extent of nucleotide variation correlated with the length of viral infection. Furthermore, no distinct subpopulations of quasispecies were detectable within an individual. The predicted amino acid sequences indicated that there were no regions within a gag protein that were comprised of clustered changes. |
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Authors:
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F K Yoshimura; K Diem; G H Learn; S Riddell; L Corey |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 70 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-01-23 Completed Date: 1997-01-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 8879-87 Citation Subset: IM; X |
Affiliation:
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Department of Biological Structure, University of Washington, Seattle 98195, USA. fyoshi@med.wayne.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U29242; U29246; U29247; U29248; U29249; U29250; U29251; U29252; U29253; U29254; U29255; U29256; U29257; U29258; U29259; U29260; U29261; U29262; U29263; U29264; U29265; U29403; U29404; U29405; U29406; U29407; U29408; U29409; U29410; U29411 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence DNA, Viral Genes, gag* Genetic Variation* HIV Infections / blood, virology* HIV-1 / classification, genetics*, isolation & purification Humans Molecular Sequence Data Phylogeny Sequence Homology, Amino Acid Virion |
| Grant Support | |
ID/Acronym/Agency:
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AI05065/AI/NIAID NIH HHS; AI27757/AI/NIAID NIH HHS; AI36613/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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