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Intrapartum polymerase chain reaction for detection of group B streptococcus colonisation.
MedLine Citation:
PMID:  23316860     Owner:  NLM     Status:  Publisher    
AIMS: Lower vaginal swabs were collected to compare the intrapartum polymerase chain reaction (PCR) test to the standard antepartum culture for detection of group B streptococcus (GBS) colonisation. MATERIALS AND METHODS: Four hundred and forty-five pregnant women with documented antepartum GBS cultures, who did not receive either antepartum or intrapartum antibiotics, were included in this prospective study. At the time of delivery and before the intrapartum antibiotic prophylaxis (IAP), double swabs were collected, one of the swab was used in the GBS molecular-based (PCR) test and the other was processed for GBS culture in Ahmadi Hospital Clinical Laboratory. Neonates were examined after delivery to diagnose GBS sepsis, and using intrapartum GBS culture as gold standard test; the intrapartum PCR test was compared to the antepartum culture. RESULTS: The sensitivity and specificity of antepartum culture to diagnose GBS colonisation were 73 & 95.5%, respectively, compared with 98.3% sensitivity & 99% specificity for intrapartum PCR test (P > 0.05). Although the intrapartum PCR test was more accurate (98.8%) for detection of GBS colonisation than antepartum culture (90%), its accuracy was significantly different. The neonatal GBS sepsis was significantly related to positive intrapartum cultures, and intrapartum PCR tests, but was not related to positive antepartum cultures (the IAP to the antepartum GBS-positive mothers reduces GBS sepsis of their neonates). DISCUSSION: The intrapartum PCR test is an accurate bedside test and it has the potential to be used as intrapartum screening for GBS colonisation allowing appropriate management of mothers and neonates.
Ibrahim A Abdelazim
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-15
Journal Detail:
Title:  The Australian & New Zealand journal of obstetrics & gynaecology     Volume:  -     ISSN:  1479-828X     ISO Abbreviation:  Aust N Z J Obstet Gynaecol     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0001027     Medline TA:  Aust N Z J Obstet Gynaecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Author ANZJOG © 2013 The Royal Australian and New Zealand College of Obstetricians and Gynaecologists.
Department of Obstetrics & Gynaecology, Ain Shams University, Cairo, Egypt; Ahmadi Hospital, Kuwait Oil Company (KOC), Ahmadi, Kuwait.
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