Document Detail


Intraoperative blood salvage and leukocyte depletion during liver transplantation with bacterial contamination.
MedLine Citation:
PMID:  19788448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Bacterial contamination is considered to be a contraindication for intraoperative blood salvage (IBS) during OLT. The aims of this study were to evaluate the efficiency of the autotransfusion device with an additional leukocyte depletion filter (LDF) for eliminating bacterial contaminations, and its clinical outcomes in terms of post-operative infections during OLT. METHODS: Forty-five patients with end-stage liver disease and cirrhotic ascites were enrolled in this study. The blood from the surgical field was collected and processed by an autotransfusion device (Cell Saver 5) and a LDF for bacteriological analysis. Among them, 12 patients with chronic severe hepatitis B received autologous transfusion for analysis of the effect on post-operative infections. RESULTS: Spontaneous bacterial peritonitis (SBP) (p < 0.05, OR = 20.1) and a long duration of operation (p < 0.01, OR = 8.3) were found to be critical risk factors for contamination. Autotransfusion devices with an additional LDF significantly eliminated bacterial contaminants from shed blood (p < 0.05). About 33% (4/12) of the patients who received autologous transfusion with salvaged and filtered erythrocytes got post-operative bacterial infection. CONCLUSIONS: Autotransfusion devices with an additional LDF could significantly eliminate bacterial contaminants of shed blood during OLT. The new mode of IBS might be a good option in reducing post-operative infections, and deserves a large-scale clinical trial.
Authors:
Ting-Bo Liang; Jun-Jian Li; Dong-Lin Li; Liang Liang; Xue-Li Bai; Shu-Sen Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-24
Journal Detail:
Title:  Clinical transplantation     Volume:  24     ISSN:  1399-0012     ISO Abbreviation:  Clin Transplant     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-07     Completed Date:  2010-08-24     Revised Date:  2010-11-10    
Medline Journal Info:
Nlm Unique ID:  8710240     Medline TA:  Clin Transplant     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  265-72     Citation Subset:  IM    
Affiliation:
Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Multi-organ Transplantation of Ministry of Public Health, Key Laboratory of Organ Transplantation of Zhejiang Province, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Blood Transfusion, Autologous / contraindications,  instrumentation*,  methods
Enterococcus faecium / isolation & purification
Escherichia coli / isolation & purification
Hepatitis B / therapy
Humans
Intraoperative Period
Klebsiella pneumoniae / isolation & purification
Leukocyte Reduction Procedures*
Liver Cirrhosis / surgery
Liver Transplantation / adverse effects,  methods*
Logistic Models
Peritonitis / epidemiology
Risk Factors
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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